Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis, including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes ...

ricin, the toxic lectin extracted from the castor bean plant (ricinus communis), consists of an a chain (rta) and a b chain (rtb). anti-a chain abs and anti-b chain abs can neutralize toxins in vivo and in vitro via blocking the binding of the toxin to the cell. also, rtb protein is able to serve as an antigen deliver to the mucosal immune system and act as an immunoadjuant. here, the genomic d...

background mycobacterium tuberculosis is a vicious microbe co-existing with the infected host. this pathogen exploited opportunities to spread during periods of urbanization and social upheaval, and got retreated with improved hygiene. objectives this investigation was designed to clone and characterize m. tuberculosis mutt gene, a homologue of a dna repair protein in escherichia coli. the aim ...

E. coli is the expression system of choice and a substantial body of literature has accumulated on the successful expression of foreign genes in this host. Several problems with protein expression in E. coli have been encountered, and many have been ultimately solved. This unit describes methods that have been developed for production of recombinant proteins in E. coli and potential pitfalls th...

In recent years, the number of recombinant proteins used for therapeutic applications and industry has increased dramatically. Recombinant proteins are produced in many host organisms (microbial, insect, plant and mammalian cells). There are many factors to consider when choosing the optimal system for protein expression and purification including the mass, purity or solubility of the recombina...

The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escherichia...

HIGHLIGHTS E. coli BL21 as a suitable expression and delivery vaccine system; rSeM protein protect mice against S. equi infection; cells enhance the immune response equi; protein, purified or not, presented immunogenicity in horses.

conclusions in conclusion, we developed a method to express the papg.acma protein on the surface of lactobacillus. this is the first report on the successful application of lactic acid bacteria displaying the papg.acma fusion protein. it will be interesting to determine the immune responses against the papg protein in near future using this surface display strategy. results the papg.acma fusion...

dna coding for the core antigen from hepatitis b virus (hbcag) was amplified, cloned and propagated in e. coli. the core protein was expressed in e. coli and the product was readily detected by western blot. this protein can be used as a diagnostic material in serum screening tests. to increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

the present work was conducted to compare the occurrence of escherichia coli possessing virulence and esbl genes in backyard and farmed poultry. three hundred and sixty samples from the poultry kept in backyard system and 120 samples from the farmed birds were collected from west bengal, india. among the e. coli isolates of backyard poultry (o2, o10, o25, o55, o60, o106, ut), none of them posse...