In this study, immunohistochemistry was used to clarify the early stages of viral kinetics and cycliccourse of IBDV, IR499, which has been described earlier as a very virulent strain (vvIBDV). Fifteen, 4-week-old SPF chickens were inoculated with 10³ EID50 of vvIBDV, IR499, via oculo/nasal route. Five birdsserved as controls, and inoculated with phosphate buffered saline (PBS). The birds were t...

in this study, immunohistochemistry was used to clarify the early stages of viral kinetics and cycliccourse of ibdv, ir499, which has been described earlier as a very virulent strain (vvibdv). fifteen, 4-week-old spf chickens were inoculated with 10³ eid50 of vvibdv, ir499, via oculo/nasal route. five birdsserved as controls, and inoculated with phosphate buffered saline (pbs). the birds were t...

In this study, susceptibility to H5N1 virus infection was studied in two Indian native chicken breeds viz. Kadaknath and Aseel (Peela) and an Indian synthetic broiler strain (Synthetic dam line (SDL-IC). Fifty birds from each genetic group were infected intra-nasally with 1000 EID50 of a highly pathogenic avian influenza virus (HPAIV) strain A/chicken/Navapur/India/7972/ 06 (H5N1) and observed ...

Background: Swine influenza viruses (SIVs) of H1N2 subtype have recently become established in several European countries, and they cocirculate with H1N1 and H3N2 viruses. The H1N2 virus haemagglutinin (HA) appears to be of human origin and fails to cross-react with avian-like H1N1 SIVs in vitro. This study examines whether in vivo cross-protection occurs between H1N1 and H1N2 viruses isolated ...

Frequent vaccination failures have occurred in the broiler farms in Eurasian countries during Newcastle disease outbreaks. The disease is enzootic in many countries of the region, especially in southwest Asia. I-2 vaccine has been used successfully in village chickens in many Asian and African countries. Our preliminary study showed good efficacy of the vaccine in broiler chickens. Therefore th...

Background: Non-ionic surfactant, Tween-80 (TW80) is commonly used for drug delivery due to its effect on the cell membrane permeation. The change in permeability can also increase viral infectivity in cells. This study was undertaken to improve upon Newcastle disease virus (NDV) titer cultivated with embryonic chicken eggs. Methods: The toxicity of TW80 was investigated against chicken embryo...

Knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. The present study aimed to evaluate the best harvesting time of H9N2 avian influenza virus (AIV) vaccine strain inoculated in specific pathogen free (SPF) embryonated chicken eggs (ECE)s. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated into 336, 11-day old ECE...

Background & Aims: The H9N2 avian influenza subtype is endemic in many parts of Iran and has the ability to transmit from bird to human. In the present study, the risk of this subtype for humans was assessed by determination of the viral titer and shedding time in bird. Method: The experiment was done on fifteen male budgerigars at 4 months of age. The birds w...

Background & Aims: The H9N2 avian influenza subtype is endemic in many parts of Iran and has the ability to transmit from bird to human. In the present study, the risk of this subtype for humans was assessed by determination of the viral titer and shedding time in bird. Method: The experiment was done on fifteen male budgerigars at 4 months of age. The birds were inoculated intranasally with al...