Elastase-deficient mutants of Pseudomonas aeruginosa are less virulent than the wild type and are easily cleared from the lungs of guinea pigs. The effect of P. aeruginosa elastase on lung epithelium, however, is not yet understood. We addressed the hypothesis that breach of the epithelial barrier by elastase from P. aeruginosa allows invading organisms and toxic substances to penetrate the int...

Proteolytic degradation of extracellular matrix is thought to play an important role in many lung disorders. In the current study, human lung fibroblasts were cast into type I collagen gels and floated in medium containing elastase, cytomix (combination of tumor necrosis factor-alpha, interleukin-1beta, and interferon-gamma), or both. After 5 days, gel collagen content was determined by measuri...

STUDY OBJECTIVES Pre-elafin, also known as trappin-2, is an elastase-specific inhibitor that could be an ideal candidate for the treatment of neutrophil elastase-driven lung diseases. The inhibitory activity of pre-elafin resides in the COOH-terminal region that can be released as mature elafin. The NH(2)-terminal moiety of pre-elafin is characterized by the presence of a specific repeating seq...

Expression of the PML-retinoic acid receptor alpha (PML-RARalpha) fusion protein is the initiating genetic event for acute promyelocytic leukemia (APL), but the molecular mechanisms responsible for disease initiation are not yet clear. Several observations have suggested that early myeloid cells are uniquely susceptible to transformation by PML-RARalpha. Recently, we have shown that the early m...

Marine resources have various biological activities and their constituents are more novel than those of land organisms. Several biologically active been found in marine Recently, many studies reported that animals (MAs) can be used as functional ingredients foods or nutraceutical due to health benefits. However, no extensively investigated the cosmeceutical MAs extracts. Here, 70% ethanol extra...

Background and Aims: Nutritional bioactive natural ingredients isolated from wild edible plants, with promising effects against diabetes and/or diabetes-related diseases continue to be investigated. There is increasing interest in these substances due their protective/therapeutic potential. The purpose of the present study investigate inhibitory potential aqueous extract Eremurus spectabilis M....

We have evaluated the diagnostic value of the fecal elastase test in comparison with the secretin-pancreozymin test in the diagnosis of exocrine pancreatic insufficiency. Pancreatic elastase was measured immunologically. Immunoreactive elastase activity in spot stools from controls ranged from 136 to 4440 microgram/g; 95% of all values were within 175 to 1500 microgram/g. The elastase assay CVs...

A series of 38 plants (55 plant extracts) from New Caledonia and 22 plants (40 plant extracts) from Vanuatu (Efate and Erromango islands) were screened for xan-thine oxidase (XOD) and elastase inhibitory activity. Of the crude extracts 82% were found to possess xanthine oxidase inhibitory activity, and 23% were active against elastase, at a concentration of 50 µg/ml. The methanol extracts of Cu...

A highly specific, fluorogenic probe detects human neutrophil elastase (hNE) in activated neutrophils and Neutrophil Extracellular Traps (NETs).

The aim of this work was to study the enzymatic activities hydrolases in blood serum patients with bacterial pneumonia, pneumonia caused by SARS-CoV-2 and practically healthy volunteers who made up comparison group. A number enzymes related innate immunity were determined: lysozyme, elastase, BAPNAamidase deoxyribonuclease activity. Results: it shown that has highest level elastase lysozyme act...