Electrospun nanofibers have been used for various biomedical applications. However, electrospinning commonly produces two-dimensional (2D) membranes, which limits the application of nanofibers for the 3D tissue engineering scaffold. In the present study, a porous 3D scaffold (3DS-1) based on electrospun gelatin/PLA nanofibers has been prepared for cartilage tissue regeneration. To further impro...

Matrilin-1 (Matn1), a cartilage-specific peri-cellular and extracellular matrix (ECM) protein, has been hypothesized to regulate ECM interactions and transmit mechanical signals in cartilage. Since Matn1 knock-out (Matn1-/-) mice exhibit a normal skeleton, its function in vivo is unclear. In this study, we found that the anabolic Acan and Col2a transcript levels were significantly higher in wil...

INTRODUCTION Articular cartilage is known to exhibit strong transient mechanical behavior. When subjected to high strainrate loading, its apparent stiffness can be 10 times greater than its stiffness at equilibrium. However, the mechanism of this strain-rate dependent response is not completely understood. While some studies have shown evidence of a fluid-flow dominant mechanism of the strain-r...

Regenerative therapies for articular cartilage are currently clinically available. However, they associated with several drawbacks that require resolution. Optimizing chondrocyte expansion and their assembly, can reduce the time costs of these more importantly increase clinical success. In this study, organoids were quickly mass produced from bovine chondrocytes a new suspension protocol. This ...

Cartilage has limited self-regenerative capacity. Tissue engineering can offer promising solutions for reconstruction of missing or damaged cartilage. A major challenge herein is to define an appropriate cell source that is capable of generating a stable and functional matrix. This study evaluated the performance of culture-expanded human chondrocytes from ear (EC), nose (NC) and articular join...

OBJECTIVE To develop a biomechanical model for cartilage which is capable of capturing experimentally observed nonlinear behaviours of cartilage and to investigate effects of collagen fibril reinforcement in cartilage. DESIGN A sequence of 10 or 20 steps of ramp compression/relaxation applied to cartilage disks in uniaxial unconfined geometry is simulated for comparison with experimental data...

BACKGROUND Cartilage tissue engineering using synthetic scaffolds allows maintaining mechanical integrity and withstanding stress loads in the body, as well as providing a temporary substrate to which transplanted cells can adhere. PURPOSE This study evaluates the use of polycaprolactone (PCL) scaffolds for the regeneration of articular cartilage in a rabbit model. STUDY DESIGN Controlled l...

INTRODUCTION Autologous techniques for the reconstruction of pediatric microtia often result in suboptimal aesthetic outcomes and morbidity at the costal cartilage donor site. We therefore sought to combine digital photogrammetry with CAD/CAM techniques to develop collagen type I hydrogel scaffolds and their respective molds that would precisely mimic the normal anatomy of the patient-specific ...