نتایج جستجو برای: elisa and rt

تعداد نتایج: 16836501  

Introduction: Consumption of anabolic steroids causes damage to various tissues, including the heart. The aim of this study was to investigate the effect of resistance training (RT) on malondialdehyde (MDA) and protein carbonyl (PC) in the heart tissue of rats exposed to stanozolol (S). Methods: In this experimental study, 18 rats with mean age of 8 weeks and weight range of 150 to 200 g were ...

H. Izadi H. Keyvanfar H. Mahravani M. Salehizadeh M. Sotudeh M. Taghizadeh S.A. Ghorashi

  FMD is one of the most highly contagious diseases of animals, caused by RNA virus belong to Picornaviridae family and Aphtovirus genus. A broad host range and occurrence of FMDV as seven serotypes and also intratypic antigenic variation without clear cut demarcations, which interferes with a concept of sub typing these factors make difficult conditions to diagnosis, control and eradication of...

2006
Mustafa USTA Hikmet Murat SİPAHİOĞLU Bülent POLAT

Two methods (DAS-ELISA and RT-PCR) were compared for the detection of Prunus necrotic ringspot virus (PNRSV) in woody host Prunus mahleb isolated from Malatya. Total RNA extractions were made from serially diluted fresh leaf tissue, root, bark and one year old green bark tissue using silica-based method. Purified total RNA extracts were used as template for cDNA synthesis by reverse transcripti...

2015
Ngoc Hai Nguyen Thi Hong Vi Vuong Tan Hung Vo

Genotyping 42 serum samples taken from the pigs in the oubreaks from 2009 to 2013 with RT-PCR and nested RT-PCR, showed more than 80% of samples were positive with Chinese PRRSV clade, and the others were European and North American classical PRRSV genotypes. Ten serum samples from unnapprent PRRS herds were examined for antibodies against PRRSV with ELISA and also for PRRSV with RT-PCR. It was...

Journal: :Acta virologica 2014
G J Hu Y F Dong Z P Zhang X D Fan R Fang H J Zhu

The presence of grapevine virus B (GVB) was detected in 188 grapevine samples from China by double antibody sandwich ELISA (DAS-ELISA) and reverse transcription-PCR (RT-PCR). The accuracy of detection by RT-PCR was confirmed by sequencing amplified PCR fragments. Seventeen samples were GVB-positive by DAS-ELISA and five by RT-PCR. The isolate QMW proved to be positive by RT-PCR only, and four i...

Journal: :Iraqi journal of Veterinary Sciences 2021

Foot-and-mouth disease (FMD) is an extremely contagious viral affects all cloven- hoofed animals. The present study aimed to investigate the epidemiological situation of FMD in Egypt during 2017 and 2018, based on antigenic genetic characterizations virus (FMDV). Thirty oral epithelia were collected from vaccinated animals (14 native cattle 16 water buffaloes) showed clinical signs four Egyptia...

Fatemeh Hamid, Leila Etemad, Mohammad Moshiri,

Seeds of the castor bean plant Ricinuscommunis L (CB) contain ricin toxin (RT), one of the most poisonous naturally-occurring substances known. Ricin toxin, a water-soluble glycoprotein that does not partition into the oil extract, is a ribosome-inactivating toxin composed of two chains, labeled A and B. Severity of the toxicity varies depending on the route of exposure to the toxin. Inhalation...

BACKGROUND: Bovine coronavirus (BCoV) is a primary cause of neonatal calf diarrhea worldwide, and is also associated with acute diarrhea in adult cattle during the winter season, resulting heavy economic losses to both dairy and beef industry throughout the world. OBJECTIVE: The Objective of the present study was to screen the fecal samples for BCoV collected from diarrhea from six geographic r...

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