Embryo culture were used to produce inter specific walnut hybrid plants between Juglans nigra L. and J. regia L. Developed ovaries were collected from J. rejia female flowers which were pollinated with J. nigra pollen grain by high dusting on a mature tree. In order to isolate embryos, exocarps were aseptically removed. Isolated embryos from ovules at different developmental stages were transfe...

Establishment of efficient protocol for the induction of somatic embryogenesis and plantlet regeneration from shoot tip explants was achieved in Glycine max (L).Merr. cv.PK472. Highest frequency (95%) of embryogenic callus formation was obtained from shoot tip explants of Glycine max (L).Merr.cv.PK472 on Murashige and Skoog (MS) medium fortified with different concentration of 2,4-Dichloropheno...

BACKGROUND PreImplantation Factor (PIF), a novel peptide secreted by viable embryos is essential for pregnancy: PIF modulates local immunity, promotes decidual pro-adhesion molecules and enhances trophoblast invasion. To determine the role of PIF in post-fertilization embryo development, we measured the peptide's concentration in the culture medium and tested endogenous PIF's potential trophic ...

BACKGROUND High concentration of glucose in culture medium affects the developmental process and the quality of the pre-implantation embryo. This study examined the effects of Zuogui Wan (ZGW) supplementation on early embryo development cultured in high-glucose medium. METHODS Embryos were cultured in high-glucose medium with or without ZGW supplementation. Developmental rate and competence w...

The purpose of the research study is to select the best rice explant and variety for Agrobacterium mediated genetic transformation studies. In vitro callus induction and regeneration frequency of two explants i.e. immature embryo and mature grains of 9 rice varieties (Oryza sativa L.) was investigated by culturing explants source on N6 media supplemented with 2, 4-D at 2mg/l for callus inductio...

The present study establishes a regeneration protocol and optimizes conditions for Agrobacterium-mediated transformation of the tetraploid emmer wheat, Triticum dicoccum. Regeneration from mature and immature embryos was accomplished as a two-step process involving callus induction in the presence of 2,4-D followed by regeneration on a 2,4-D free, cytokinin-containing medium (RM1). Higher conce...

Somatic embryogenesis in Arabidopsis is achieved by culturing bending-cotyledon embryos on a 2,4-D-containing induction medium for 14 d followed by a transfer on to a hormone-free development medium. Several genes orthologous to Arabidopsis SHOOTMERISTEMLESS (STM), CLAVATA 1 (CLV1), and ZWILLE (ZLL) were isolated from Brassica oleracea (Bo), B. rapa (Br), and B. napus (Bn), and ectopically expr...

The neural crest gives rise to numerous derivatives including adrenergic and cholinergic neurons, supportive cells of the nervous system, and melanocytes. In tissue culture, neural crest cells explanted from both cranial and trunk regions were found to differentiate into adrenergic neuroblasts or into pigmented cells when grown in medium containing 10% chick embryo extract. When the embryo extr...