Given the complex nature of Alzheimer's disease (AD), a cell-based model that recapitulates the physiological properties of the target neuronal population would be extremely valuable for discovering improved drug candidates and chemical probes to uncover disease mechanisms. We established phenotypic neuronal assays for the biogenesis and synaptic action of amyloid β peptide (Aβ) based on embryo...

The current morphologically based selection of human embryos for transfer cannot detect chromosome aneuploidies. So far, only biopsy techniques have been able to screen for chromosomal aneuploidies in the in vitro fertilization (IVF) embryos. Preimplantation genetic diagnosis (PGD) or screening (PGS) involves the biopsy of oocyte polar bodies or embryonic cells and has become a routine clinical...

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...

Meiotic errors during oocyte maturation are considered the major contributors to embryonic aneuploidy and failures in human IVF treatment. Various technologies have been developed to screen polar bodies, blastomeres and trophectoderm cells for chromosomal aberrations. Array-CGH analysis using bacterial artificial chromosome (BAC) arrays is widely applied for preimplantation genetic diagnosis (P...

among the female sexual hormones, the estrogen and progesterone are of significant importance. these hormones are prescribed for the treatment of certain female genital system disorders as well as used in the synthesis of contraceptive drugs. it has been shown that administration of the above hor mones during early pregnancy may produce abortion in early gestational days. in the present investi...

embryonic stem cells (escs) are originally derived from the icm of blastocysts and are characterized by their ability to self-renew and their pluripotencies. only a few reports have been published on esc isolations and line establishment in animals, even fewer in horses. however, it is still important to isolate equine escs for animal biotechnology and therapeutic applications. in the present s...

in spite of serious cardiotoxicity side-effects, doxorubicin is frequently used for treatment of several types of cancers. isolated human adult cardiomyocytes could be the best model for assessing drug-induced cardiotoxicity, while harvesting mature cardiomyocytes is restricted by some limitations such as biopsy size, cell numbers, viability, proliferative capacity and their disability to be pa...

embryonic stem cells (escs) are pluripotent, self-renewing cells. these cells can be used in applications such as cell therapy, drug discovery, disease modelling, and the study of cellular differentiation. in this experimental study embryonic stem cells cultured in the laboratory and were amplified. total rna was extracted from cells and converted to cdna by reverse transcription-polymerase cha...

In recent years, the concept of precision medicine—an approach to developing personalized drugs for disease prevention or treatment—has emerged as an up-and-coming field in medical research. However, there are numerous limitations applying this treatment neurodegenerative diseases, such Alzheimer disease, because invasiveness obtaining human brain samples. Meanwhile, development organoids has b...