The balance between oxidation and antioxidation is believed to be critical in maintaining healthy biological systems. Under physiological conditions, the human antioxidative defense system including e.g., superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione (GSH) and others, allows the elimination of excess reactive oxygen species (ROS) including, among others s...

Assisted reproductive technology (ART) is a common treatment of choice for many couples facing infertility issues, be it due to male or female factor, or idiopathic. Employment of ART techniques, however, come with its own challenges as the in vitro environment is not nearly as ideal as the in vivo environment, where reactive oxygen species (ROS) build-up leading to oxidative stress is kept in ...

Exercise promotes longevity and ameliorates type 2 diabetes mellitus and insulin resistance. However, exercise also increases mitochondrial formation of presumably harmful reactive oxygen species (ROS). Antioxidants are widely used as supplements but whether they affect the health-promoting effects of exercise is unknown. We evaluated the effects of a combination of vitamin C (1000 mg/day) and ...

The interplay between free radicals, antioxidants, and co-factors is important in maintaining health, aging and age-related diseases. Free radicals induce oxidative stress, which is balanced by the body's endogenous antioxidant systems with an input from co-factors, and by the ingestion of exogenous antioxidants. If the generation of free radicals exceeds the protective effects of antioxidants,...

SIGNIFICANCE It is generally accepted that reactive oxygen species (ROS) scavenging molecules or antioxidants exert health-promoting effects and thus their consumption as food additives and nutraceuticals has been greatly encouraged. Antioxidants may be beneficial in situations of subclinical deficiency and increased demand or acutely upon high-dose infusion. However, to date, there is little c...

Peroxidases are very important enzymes, e.g., as preventive antioxidants by removing noxious peroxides from the blood. For this reason we evaluated a colorimetric method which detects the activity of endogenous peroxidases by their reaction with hydrogen peroxide, using tetramethylbenzidine as the chromogenic substrate. This assay design can be easily reversed by change of the variable compound...

Mol. Nutr. Food Res. 2020, 65, 1901071 DOI: 10.1002/mnfr.201901071 Dietary stressors, including saturated fats and sugars, generate endogenous stress signals that reconfigure host metabolism towards a glycolytic pro-inflammatory state. Conversely, exercise, unsaturated fats, dietary antioxidants direct oxidative phosphorylation lower reactive oxygen species. This metabolic environment promotes ...

In this study, total phenolic contents and antioxidant activity were determined in various products made from two cultivars of jamman fruit (Eugenia jambolana) i.e. improved (V1) indigenous (V2). Five each cultivar such as jam, squash, ready-to-drink juice, pulp powder seed powder. Fruit endogenous to the typical region Sindh-Pakistan. Total analyzed by Folin-Ciocalteu colorimetric method, wher...