Although currently available strategies for the preparation of exome-enriched libraries are well established, a final validation of the libraries in terms of exome enrichment efficiency prior to the sequencing step is of considerable importance. Here, we present a strategy for the evaluation of exome enrichment, i.e., the Multipoint Test for Targeted-enrichment Efficiency (MTTE), PCR-based appr...

The incidence of belly nosing-related behaviors was studied in 480 piglets weaned at 14 d and provided with environmental enrichment during preweaning, postweaning, or both. Pen enrichment was achieved by providing a foam rubber mat on the pen wall (nose), rubber nipples (suck), a Bite-Rite Tail Chew (bite), a soil-filled tray (root), or no enrichment (control). Instantaneous scan sampling obse...

Environmental conditions such as housing and husbandry have a major impact on the laboratory animal throughout its life and will thereby influence the outcome of animal experiments. However, housing systems for laboratory animals have often been designed on the basis of economic and ergonomic aspects. One possible way to improve the living conditions of laboratory animals is to provide opportun...

eight selected heavy metals (cu, zn, pb, cd, cr, mn, fe and al) in surface and sub-surfacesediments in the northern coast of sfax (tunisia) were studied in order to assess the sediment quality and tohighlight the anthropogenic contributions to heavy metal distributions in the two study sediment levels.multiple chemometric approaches based on geographic information system (gis), enrichment facto...

A comparison of various methods of enhancing frequency of Salmonella isolations revealed that inoculation of a second enrichment broth, with culture from the first, was no improvement over the single direct enrichment method. It was inferior to centrifugation. Selenite was observed to produce more positive isolations at 48 hr than at 24. No change occurred in tetrathionate. Reconstitution of dr...

Environmental enrichment for laboratory animals is a widely accepted practice for many species, but few studies address the periods of preadolescence and adolescence. Provision of igloos, tunnels, nesting materials, and social or communal housing are commonly used enrichment strategies in rat cages. In the present study, the effects of individual, pair, and trio housing and the presence or abse...

The cognitive reserve hypothesis helps to explain the incomplete relationship between brain disease and cognitive status in people with neurologic diseases, including Alzheimer's; disease and multiple sclerosis. Lifetime intellectual enrichment (estimated with education or vocabulary knowledge) lessens the negative impact of brain disease on cognition, such that people with greater enrichment a...

background: the mainstay of diagnosis of relapsing fever (rf) is demonstration of the spirochetes in giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by mi­cros­copy. this study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spi­ro­chetemia. methods: animal blood sampl...

this study was accomplished to investigate the effect of artemia urmiana nauplii enriched with pediococcus acidilactici as probiotic on growth performance and survival rate of great sturgeon, huso huso.  artemia nauplii were enriched with p. acidilactici at a final concentration of 1010 cfu ml-1 in three time dependent treatments as 3 h (t3), 6 h (t6), 9 h (t9), and one non-enriched artemia as ...