background: in algeria, date palm is currently confronted to the bayoud disease. biotechnological tools such as protoplasts fusion can appear as an alternative to ensure rapid multiplication and improvement of this species.objectives: callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars.materials and methods: some factors influencing the isolation ...

We demonstrate a novel assay for purification of RNA (16S rRNA) from whole human blood infected with Pseudomonas Putida (P. Putida). This assay is unique in that the extraction chemistry provides a method which both lyses bacteria cells and protects RNA from degradation when isolating from matrices rich with ribonuclease (RNase) (such as blood). The method can also be integrated with on-chip RN...

Chitosan is a naturally occurring polysaccharide with antimicrobial properties. This study investigated whether chitosan could induce the LytSR two-component autolytic regulatory system, specifically the cid and lrg operons, found in Streptococcus mutans. A turbidity assay and a murein hydrolase assay were used to observe autolytic activity in chitosan-treated and non-treated cultures of wildty...

background : microbial monitoring of aquatic sources is essential in the correct management of these vital sources. multiple tube fermentation (mtf) technique is currently used for assessment of the microbial quality of water sources. however, today enzymatic assay has been presented as an alternative approach for detecting indicator bacteria. the purpose of this study was to compare enzymatic ...

in current study, protein hydrolysates were produced using microbial proteases of alcalase, protamex and flavourzyme from yellowfin tuna viscera. the results indicated that, protein hydrolysate from alcalase, had significantly higher protein content, nitrogen recovery and degree of hydrolysis than the others (p 0.05). the results of amino acids composition revealed that all hydrolysates were al...

esterification of chitosan with adipic acid catalyzed by immobilized candida antarctica lipase b was carried out in this study. response surface methodology (rsm) based on a four-factor- five-level small central composite design (sccd) was employed to model and analyze the reaction. a total of 21 experiments representing different combinations of the four reaction parameters including ch...

the etiopathogenesis of alzheimer's disease (ad) is still unclear.  however, long-term oxidative stress is believed to be one of the major contributing factors in progression of neuronal degeneration and decline of cognitive function in ad. in order to assess the presence of oxidative stress in ad, we examined the enzymatic activities of the erythrocyte cu-zn superoxide dismutase (cu-zn sod), g...