A bicatalytic system was prepared by the immobilization of alcohol oxidase enzyme and an alkylamine organocatalyst in distinct locations of mesoporous nanoparticles. The resulting nanocomposites were able to perform a sequence of oxidation and aldol coupling reactions, which transformed short chain alcohols into longer chain molecules. The process takes place at low temperatures, but requires a...

Most drugs function by binding reversibly to specific biological targets, and therapeutic effects generally require saturation of these targets. One means of decreasing required drug concentrations is incorporation of reactive metal centers that elicit irreversible modification of targets. A common approach has been the design of artificial proteases/nucleases containing metal centers capable o...

Naturally occurring enzymes are remarkable biocatalysts with numerous potential applications in industry and medicine. However, many of their catalyst properties often need to be further tailored to meet the specific requirements of a given application. Within this context, directed evolution has emerged over the past decade as a powerful tool for engineering enzymes with new or improved functi...

within the past few years, neat scheme has been overturned by the discovery that rna can act as an enzyme. what does the starting finding of rna enzymes imply? thre first implication is that one can no longer assume a protein lies behind every catalytic activity of the cell. it now appears that several of the operations that tailor an ran molecule into its final form are at least in part cataly...

A living organism must not only organize itself from within; it must also maintain its organization in the face of changes in its environment and degradation of its components. We show here that a simple (M,R)-system consisting of three interlocking catalytic cycles, with every catalyst produced by the system itself, can both establish a non-trivial steady state and maintain this despite contin...

We report here a molecular modeling investigation of steroidal and nonsteroidal inhibitors of human cytochrome P450 17alpha-hydroxylase-17,20-lyase (CYP17). Using the pharmacophore perception technique, we have generated common-feature pharmacophore model(s) to explain the putative binding requirements for two classes of human CYP17 inhibitors. Common chemical features in the steroid and nonste...

The completely selective oxidation of toluene to benzaldehyde with dioxygen, without the need use H2O2, halogens, or any radical initiators, is a reaction long desired but never previously successful. Here, we demonstrate enzyme-like mechanism over hexadecylphosphate acid (HDPA)-bonded nano-oxides, which appear interact through specific recognition. active sites catalyst are related ability HDP...

We present a study of electrocatalysis by an enzyme adsorbed on a range of carbon materials, with different size, surface area, morphology and graphitic structure, which are either commercially available or prepared via simple, established protocols. We choose as our model enzyme the hydrogenase I from E. coli (Hyd-1), which is an active catalyst for H2 oxidation, is relatively robust and has b...

Tailoring conducting polymers (CPs) such as polyaniline (PANI) to deliver the appropriate morphology, electrochemical properties and processability is essential for development of effective polymer-based sensors biosensors. Composite PANI electrodes detection ammonium (NH4+) have been previously reported, but limited by their reliance on electrocatalytic reaction between NH4+ a metal/nano-catal...

Enantioselective production of (S)-2-phenyl-1-propanol is important as in order to be applied industry, a high degree optical purity required. Besides organocatalysts and metal complexes, biocatalysts can used for its synthesis their isolated form or whole-cell biocatalysts, both which have various advantages disadvantages. In this research, Saccharomyces cerevisiae, biocatalyst, recombinant ho...