Rheumatoid factor (RF) is a major source of interference in many immunoassays. Most immunoassays use mammalian polyclonal or monoclonal antibodies, and RF can react with IgG from mammalian species, thus causing false-positive results. In this work we have studied RF interference in a sandwich ELISA, where RF in the sample may react with both the capture antibody and the detection antibody to gi...

Currently, great significance is attached to the preparation of diagnostic drugs based on specific immunoreagents, which include monoclonal antibodies produced by hybridomas. The use one important approaches for detection pathogens particularly dangerous infections – anthrax, brucellosis, tularemia, plague, cholera, glanders, and melioidosis. review presents main achievements Russian scientists...

The rate-limiting step in many solid-phase immunoassays is associated with the slow kinetics of binding of macro-molecular antigen and conjugate to the immobilized phase. We demonstrate that the use of ultrasonic energy to enhance mass transport across liquid/solid interfaces can dramatically accelerate antigen binding to immobilized antibodies. We use an ultrasound-accelerated procedure with a...