نتایج جستجو برای: epitope maping

تعداد نتایج: 19160  

Journal: :Vaccine 2013
Hiep L X Vu Byungjoon Kwon Marcelo de Lima Asit K Pattnaik Fernando A Osorio

DIVA (differentiating infected from vaccinated animals) vaccines have proven extremely useful for control and eradication of infectious diseases in livestock. We describe here the characterization of a serologic marker epitope, so-called epitope-M201, which can be a potential target for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus (PR...

Journal: :PLoS Pathogens 2008
Sofia Marques Marta Alenquer Philip G. Stevenson J. Pedro Simas

The pathogenesis of persistent viral infections depends critically on long-term viral loads. Yet what determines these loads is largely unknown. Here, we show that a single CD8+ T cell epitope sets the long-term latent load of a lymphotropic gamma-herpesvirus, Murid herpesvirus-4 (MuHV-4). The MuHV-4 M2 latency gene contains an H2-Kd -restricted T cell epitope, and wild-type but not M2(-) MuHV-...

Journal: :Structure 2010
Bruno E Correia Yih-En Andrew Ban Margaret A Holmes Hengyu Xu Katharine Ellingson Zane Kraft Chris Carrico Erica Boni D Noah Sather Camille Zenobia Katherine Y Burke Tyler Bradley-Hewitt Jessica F Bruhn-Johannsen Oleksandr Kalyuzhniy David Baker Roland K Strong Leonidas Stamatatos William R Schief

Broadly cross-reactive monoclonal antibodies define epitopes for vaccine development against HIV and other highly mutable viruses. Crystal structures are available for several such antibody-epitope complexes, but methods are needed to translate that structural information into immunogens that re-elicit similar antibodies. We describe a general computational method to design epitope-scaffolds in...

Ehsan Aryan, Hamed Gouklani, Kobra Salimiyan Rizi, Zahra Meshkat,

Background: Today, several vaccines have been developed to prevent infectious diseases. The older first-generation vaccines may have many problems. In this regard, genetic engineering plays an important role using tools such as shuttle vectors to develop recombinant DNA vaccines that usually include plasmid constructed so that can propagate in two different host species. The present study revie...

Journal: :BioTechniques 1997
C Hatfield K M Duus J Chen D H Jones C Grose

We describe a rapid PCR method that directly inserts an epitope tag into an open reading frame (ORF) to facilitate protein detection. This project was performed within a varicella-zoster virus (VZV) system. In earlier work, we produced a monoclonal antibody (MAb 3B3) to one VZV ORF called gE. MAb 3B3 bound to its epitope under extreme denaturing conditions. To further characterize the epitope, ...

Journal: :Cancer research 2000
T D Schell B B Knowles S S Tevethia

The role of CTL tolerance in tumor immunity to SV40 large T antigen (T ag)-induced tumors was studied using T ag transgenic mice of the line 501 (H2b). 501 mice express SV40 T ag under the influence of the alpha-amylase promoter, which leads to the development of osteogenic osteosarcomas late in life and eventual death between 12 and 17 months of age. We determined the ability of 501 mice to re...

2000
Todd D. Schell Barbara B. Knowles Satvir S. Tevethia

The role of CTL tolerance in tumor immunity to SV40 large T antigen (T ag)-induced tumors was studied using T ag transgenic mice of the line 501 (H2). 501 mice express SV40 T ag under the influence of the a-amylase promoter, which leads to the development of osteogenic osteosarcomas late in life and eventual death between 12 and 17 months of age. We determined the ability of 501 mice to respond...

Journal: :Blood 2021

Abstract Platelet transfusion refractoriness results in adverse outcomes and increased health care costs. Managing resulting from HLA alloimmunization necessitates the use of antigen–matched platelets but requires a large platelet donor pool does not guarantee full matching. We report first randomized, double-blind, noninferiority, crossover trial comparing epitope–matched (HEM) with standard (...

2016
Naga Bhushana Rao .K Ranjit Prasad Bahadur

Characterization of B-cell protein epitope and developing critical parameters for its identification is one of the long standing interests. Using Layers algorithm, we introduced the concept of anchor residues to identify epitope. We have shown that majority of the epitope is composed of anchor residues and have significant bias in epitope for these residues. We optimized the search space reduct...

2002
Tong Ihn Lee Nicola J. Rinaldi François Robert Duncan T. Odom Ziv Bar-Joseph Georg K. Gerber Nancy M. Hannett Christopher T. Harbison Craig M. Thompson Itamar Simon Julia Zeitlinger Ezra G. Jennings Heather L. Murray D. Benjamin Gordon Bing Ren John J. Wyrick Jean-Bosco Tagne Thomas L. Volkert Ernest Fraenkel David K. Gifford Richard A. Young

Epitope Tagging of Strains Regulators were tagged by inserting multiple copies of the Myc epitope coding sequence into the normal chomosomal loci of these genes. The plasmid pWZV88 (1) was used as a template to generate PCR products containing the Myc epitope coding sequence and a selectable marker (TRP) flanked by homologous regions designed to recombine at the 3’ end of the targeted transcrip...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید