rehearsed routines had to be changed. The result: an unanticipated mishap about a week after the new process was implemented. During morning rounds, a supplemental IV phosphate bolus had been prescribed for a critically ill 12-year-old child with a low phosphate level. Later that evening, the child’s phosphate level was extremely high. Despite aggressive therapy to correct the abnormal electrol...

Using freshly precipitated manganese-diethyldithiocarbamate (Mn(DDTC)(2)) as a new reagent, a solid phase extraction method (SPE) has been developed for the extraction of Cd(II) and Zn(II) in aqueous fertilizer samples. A sample solution of 300 mL was taken and 0.10 g of freshly precipitated Mn(DDTC)(2) was added. After adding a phosphate buffer solution, the mixture was stirred at 10 min, filt...

A method to prepare photosystem I (PSI) particles is described. Spinach leaves are used to prepare broken chloroplasts that are then solubilized by using a detergent (Triton X-100). Solubilized chloroplasts are then applied on an ion-exchange column. Eluted by a linear concentration gradient of NaCl, fractions enriched in PSI particles are collected and applied on a small hydroxyapatite column....

A highly sensitive fluorimetric assay for the measurement of pyridoxal and pyridoxal phosphate in biological tissues is described. The method involves the enzymic hydrolysis of pyridoxal phosphate to pyridoxal. The pyridoxal (free or total) is separated on an anion-exchange column, concentrated by cation-exchange chromatography and reacted with potassium cyanide under slightly alkaline conditio...

Newton & Abraham (1950) found that crude bacitracin could be resolved into a series of similar polypeptides by counter-current distribution between solvents. Better resolution and larger amounts ofthe products have now been obtained by using a greater number of transfers and starting with more concentrated solutions than were used in the earlier experiments. It has been possible, by a single di...

Inactivation of Giardia lamblia and Giardia muris cysts was compared by using an ozone demand-free 0.05 M phosphate buffer in bench-scale batch reactors at 22 degrees C. Ozone was added to each trial from a concentrated stock solution for contact times of 2 and 5 min. The viability of the control and treated cysts was evaluated by using the C3H/HeN mouse and Mongolian gerbil models for G. muris...

Abstract In this manuscript, efficiency of the used organic solvent for recovering, separating and producing highly purified zirconium oxide from chloride leach liquor Rosetta white zircon concentrate was investigated. Zircon mineral thermally decomposed with caustic soda, cooled, washed distilled water then dissolved in hydrochloric acid. Zirconium extraction reached 91.4% using 10% bis-(2-eth...