In this study, ETo was calculated using FAO-56 Penman Monteith method. Lag-1 autocorrelation coefficient was performed to detect the presence of serially correlation in data series. Mann-Kendall (MK) or Modified Mann Kendall (MMK) and Spearman’s rho test were applied to detect the trend in annual and monthly ETo at 8 districts over 49 years (1960–2008) in the Soenath river basin, Chhattisgarh (...

Ethylene oxide (ETO) sterilization of polyvinyl trays used in an antiviral screening program was initiated to overcome seasonal outbreaks of bacterial and mycotic contamination of tissue culture cells. Trays sterilized by 100% ETO for 5 hr after 48 hr of prehumidification, or by 12% ETO plus 88% Freon 12 for 1, 3, 5 or 18 hr, followed by various methods of aeration, were seeded with several typ...

The human lysozyme (LZM) gene is highly methylated in LZM-nonexpressor immature myeloid and in nonmyeloid cells and unmethylated only in LZM-expressing cells. Extended methylation analyses of the CpG-poor 5' flanking region and of the exon 4 CpG island (both containing Alu elements) of the LZM gene were now performed. Marked demethylation was noted after treatment of AML1/ETO-positive Kasumi-1 ...

The 8;21 translocation, t(8;21)(q22;q22.3), is seen only in acute myelog<nous leukemia and is characteristically associated with the M2 subtype. Subsequent to our identification of the t(8;21) breakpoint region on chromosome 21, we reported that the translocation results in the fusion of the \\ll.l gene on chromosome 21 with a novel gene on chromosome 8 which we called ETO (for eight twenty-one...

Additional sex combs-like (ASXL) proteins are mammalian homologues of additional sex combs (Asx), a regulator of trithorax and polycomb function in Drosophila. While there has been great interest in ASXL1 due to its frequent mutation in leukemia, little is known about its paralog ASXL2, which is frequently mutated in acute myeloid leukemia patients bearing the RUNX1-RUNX1T1 (AML1-ETO) fusion. H...

Targeting of gene regulatory factors to specific intranuclear sites may be critical for the accurate control of gene expression. The acute myelogenous leukemia 8;21 (AML1yETO) fusion protein is encoded by a rearranged gene created by the ETO chromosomal translocation. This protein lacks the nuclear matrix-targeting signal that directs the AML1 protein to appropriate gene regulatory sites within...

RUNX1/ETO, the fusion protein resulting from the chromosomal translocation t(8;21), is one of the most frequent translocation products in acute myeloid leukemia. Several in vitro and in vivo studies have shown that the homo-tetramerization domain of ETO, the nervy homology region 2 (NHR2), is essential for RUNX1/ETO oncogenic activity. We analyzed the energetic contribution of individual amino ...

AML1/ETO is the chimeric protein resulting from the t(8;21) in acute myeloid leukemia. The Nervy homology 2 (NHR2) domain in ETO mediates oligomerization and AML1/ETO's interactions with ETO, MTGR1, and MTG16, and with the corepressor molecules mSin3A and HDAC1 and HDAC3. We solved the NHR2 domain structure and found it to be an alpha-helical tetramer. We show that oligomerization contributes t...

The 8;21 translocation, t(8;21)(q22;q22.3), is seen only in acute myelog<nous leukemia and is characteristically associated with the M2 subtype. Subsequent to our identification of the t(8;21) breakpoint region on chromosome 21, we reported that the translocation results in the fusion of the \\ll.l gene on chromosome 21 with a novel gene on chromosome 8 which we called ETO (for eight twenty-one...