The Pichinde virus RNA did not possess the following characteristics of eucaryotic mRNA: polyadenylic acid sequence, capped methylated structure, and ability to direct protein synthesis in vitro. Polysomal RNA extracted from cells infected with Pichinde virus reannealed with 32P-labeled virus RNA, protecting about 60% of the latter against RNase degestion. The polyadenylic acid-containing polys...

In eucaryotic cells, DNA is bound to various proteins and forms a complex nucleoprotein structure called chromatin. Indispensable and ubiquitous chromatin components are histones, small proteins of highly conserved primary structure. Two molecules of each H2A, H2B, H3 and H4 type, form the nucleosomal core around which a 146 base pair (bp) stretch of DNA is wrapped. Histone H1 is bound to about...

γ-Tubulin was initially identified as a suppressor of an Aspergillus β-tubulin mutation (Oakley and Oakley, 1989). Highly homologous genes have since been identified in a variety of other eucaryotes (see Tables 1 and 2), and immunofluorescence studies have confirmed that γ-tubulin is expressed in a wide variety of eucaryotic cells. These observations indicate that γ-tubulin is functionally impo...

Deletion mutants within the Py DNA region between the replication origin and the beginning of late protein coding sequences have been constructed and analysed for viability, early gene expression and viral DNA replication. Assay of replicative competence was facilitated by the use of Py transformed mouse cells (COP lines) which express functional large T-protein but contain no free viral DNA. V...

The mechanism(s) of interaction between Mycoplasma pulmonis and eucaryotic cells was studied by adherence to and hemagglutination of erythrocytes. Simple and complex carbohydrates and glycoproteins were unable to inhibit either adherence or hemagglutination, indicating that neither was a lectin activity. Both interactions appeared to be hydrophobic due to their requirement for salt and their se...

Three factor H mRNA species of 4.3 kb, 1.8 kb, and 1.4 kb are constitutively expressed in human liver. Having previously characterized full-length cDNA clones derived from the 4.3-kb and 1.8-kb factor mRNA, we report here the isolation and eucaryotic expression of full-length cDNA clones coding for the 1.4-kb mRNA species. The 1266-bp cDNA codes for a polypeptide of 330 amino acids and contains...