To examine whether leukotrienes could increase blood-brain barrier permeability, rats were anesthetized and injected intravenously with Evans blue. Ten microliters of vehicle, of leukotrienes B4, C4, or E4, or of arachidonic acid was injected over 1 hour directly into the brain parenchyma. The percentage of the total surface area of Evans blue extravasation in a coronal section of brain centere...

The aim of the present study was the decolourisation of mixture of two dyes belonging to different groups by two Pseudomonas fluorescens strains (Sz6 and SDz3). Influence of different incubation conditions on decolourisation effectiveness was evaluated. Dyes used in the experiment were diazo Evans blue (EB) and triphenylmethane brilliant green (BG). Another goal of the experiment was the estima...

1. Plasma volume, packed cell volume (PCV), blood pressure, extracellular fluid volume (ECFV) and evans blue disappearance rate were measured in conscious spontaneously hypertensive rats and in weight-matched Wistar normotensive rats. 2. Over the weight range studied (250-350 g), plasma and blood volumes were significantly lower in the spontaneously hypertensive rat. Extracellular fluid volumes...

Liposome is colloidal, vesicular structures capable of targeting to specific sites. Evans blue dye (hydrophilic dye) were formulated in liposomes using phosphatidyl choline, cholesterol and those liposomes were modified using surfactants surfactants span 20 , span 40 , span 80 or tween 80 at various molar ratio and optimized for its stable vesicular formation. The particle size and FTIR analysi...

The vital staining properties of rose cultures (Rosa cv Paul's Scarlet) of increasing age were compared with their ability to be subcultured. At 4-day intervals beginning on day 14, after cell division and expansion had stopped, cells were stained separately with Evans blue, fluorescein diacetate, and phenosafranine. The degree to which parent cultures stained with each of these dyes was compar...

Rapid occlusion of the middle cerebral artery (MCA) was undertaken in 5-6 week old rats to determine whether or not the young spontaneously hypertensive rat (SHR) or the normotensive Sprague-Dawley rat (SD) is protected against cerebral infarction by collateral circulation. Rats were killed 3 days after MCA occlusion and administration of Evans blue. As compared to SD, the SHR had elevated bloo...