Ureteric contractions propel foetal urine from the kidney to the urinary bladder. Here, we show that mouse ureteric smooth muscle cell (SMC) precursors express the transcription factor teashirt 3 (TSHZ3), and that Tshz3-null mutant mice have congenital hydronephrosis without anatomical obstruction. Ex vivo, the spontaneous contractions that occurred in proximal segments of wild-type embryonic u...

the present work developed an efficient plant regeneration and propagation system via direct organogenesis for morinda officinalis how., a valuable endangered medicinal plant. the effects of explant types on shoot induction, plant growth regulators on shoot proliferationand elongation and the subsequent rooting ability of shoots were examined. among the various types of explant, direct shoot pr...

PURPOSE Circadian rhythms are central to vision and retinal physiology. A circadian clock located within the retina controls various rhythmic processes including melatonin synthesis in photoreceptors. In the present study, we evaluated the rhythmic expression of clock genes and clock output genes in retinal explants maintained for several days in darkness. METHODS Retinas were dissected from ...

Two explants of shell were removed from each of several fertile eggs of domestic fowl at different times during incubation. The chorioallantoic membrane (CAM) was removed from one of the explants (SHELL ONLY) and was left in situ on the other (SHELL+CAM). Explants were cultured for 24, 48 or 96 h at 37 degrees C and 5% CO2 in air in individual Petri dishes containing Dulbecco's modified Eagle's...

An efficient protocol of common bean transformation using Agrobacterium tumefaciens is still unavailable. Several regeneration reports are available using cotyledonary node or embryonic axis as the starting explants. These explants were used for direct organogenesis exploiting the meristems for direct shoot production. Attempts were made to transform cotyledonary node explants and embryonic axe...

PURPOSE To determine whether resurfacing submacular human Bruch's membrane with a cell-deposited extracellular matrix (ECM) improves retinal pigment epithelial (RPE) survival. METHODS Bovine corneal endothelial (BCE) cells were seeded onto the inner collagenous layer of submacular Bruch's membrane explants of human donor eyes to allow ECM deposition. Control explants from fellow eyes were cul...

The Dendrobium orchid is one type with special features and many variations. This study aims to determine the administration of various concentrations ferrous sulfate (FeSO4) Thiamin sp explants on Murashige And Skoog media. design used in this was a Factorial Completely Randomized Design (CRD) consisting 2 treatment levels (F = FeSO4 T Thiamin) three replications. Namely: F0 (Without FeSO4), F...

(1) Background: Mechanical loading is an essential part of the function and maintenance joint. Despite importance intermittent mechanical loading, this factor rarely considered in preclinical models cartilage, limiting their translatability. The aim study was to investigate effects dynamic compression on extracellular matrix during long-term culture bovine cartilage explants. (2) Methods: Bovin...

The objective of this research is to lift the dormancy seeds S. kunthianum in with a view domesticating them. In experiment we used soaking sulfuric acid for different durations (10 min, 30 min and 1 h). treated were sown transparent germinators. explants obtained then transplanted into pots containing five types substrate (black earth, fine sand, sawdust, mixtures 1/1 sawdust/black earth sawdu...