In the present study, the mechanism of intercellular calcium wave propagation in bone cell networks was identified. By using micro-contact printing and self-assembled monolayer technologies, two types of in vitro bone cell networks were constructed: open-ended linear chains and looped hexagonal networks with precisely controlled intercellular distances. Intracellular calcium responses of the ce...

An important ailments that requires treatment by the physician is cardiac failure (“Heart Failure (HF)”), which usually decreases the contractility of the myocardium. The Purkinje Fiber Cells (PFC) augurs to be an important biological network of the human cardiac ventricular conduction system. It assuages to be tertiary pacemaker of the heart which synchronizes the ventricular conduction. This ...

o bjectives: the aim of this study was to assess the cytotoxic effects and osteogenic activity of recombinant human bone morphogenetic protein (rhbmp2) and nano-hydroxyapatite (n- ha) adjacent to mg-63 cell line. mat erials and methods: to assess cytotoxicity, the 4,5-dimethyl thiazolyl-2,5-diphenyl tetrazolium bromide (mtt) assay was used. alkaline phosphatase (alp) activity and oste- ogenic a...

Attachment assays of a Pseudomonas isolate to fused silica slides showed that treatment with DNaseI significantly inhibited cellular adsorption, which was restored upon DNA treatment. These confirmed the important role extracellular (eDNA) adsorption surface. To investigate eDNA mechanism, single-molecule force spectroscopy (SMFS) used measure silicon surfaces in presence different concentratio...

Simultaneous patch-clamp and fura-2 measurements were used to investigate the electrical properties and receptor-mediated changes of intracellular calcium in renal juxtaglomerular cells. Here we report the presence of voltage-activated inward and outward rectifying potassium currents and the inhibition of the anomalous inward rectifying potassium current by angiotensin II (ANG-II). This action ...

Calcium ions mediate extracellular signals on intracellular processes. The signalling system based on transient rises or oscillations of the cytoplasmic calcium concentration has potential advantages. The relevant mechanisms of intracellular concentration changes include calcium-induced calcium release and calcium dependent inactivation of calcium release. A model has been devised based on thes...

The Entamoeba histolytica upstream regulatory element 3-binding protein (URE3-BP) binds to the URE3 sequence of the Gal/GalNAc-inhibitable lectin hgl5 and ferredoxin 1 (fdx) gene promoters. This binding can be inhibited in vitro by addition of calcium. Two EF-hand motifs, which are associated with the ability to bind calcium, are present in the amino acid sequence of URE3-BP. Mutation of the se...

OBJECTIVE To review the components of calcium, phosphate and magnesium metabolism that are relevant to the critically ill patient in a two-part presentation. DATA SOURCES A review of articles reported on calcium, phosphate and magnesium disorders in the critically ill patient. SUMMARY OF REVIEW Calcium, phosphate and magnesium have important intracellular and extracellular functions with th...

Abstract Calcium is responsible for the effectiveness of various processes, and its supply in diet necessary normal function human body. Apart from being an important component skeleton, calcium also helps maintain structure cell organelles regulates intracellular extracellular fluid homeostasis. This review presents nutritional health factors that affect bioavailability calcium. Physiological ...