A rapid colony immunoblot screening procedure was used to demonstrate the surface localization of porin protein F on bacterial colonies of Pseudomonas aeruginosa. By this method, we demonstrated that protein F was accessible to four different specific monoclonal antibodies in a wide variety of both mucoid and nonmucoid P. aeruginosa strains. Controls were performed to demonstrate that, using th...

  With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...

The importance of the F protein cytoplasmic tail (CT) for replication of human respiratory syncytial virus (HRSV) was examined by monitoring the behavior of viruses expressing F proteins with a modified COOH terminus. The F protein mutant viruses were recovered and amplified under conditions where F protein function was complemented by expression of a heterologous viral envelope protein. The ef...

To evaluate the effect of calcium salts long-chain fatty acids (CaLCFA) and protein supplementation on body weight (BW), average daily gain (ADG), pregnancy rate (PR), blood chemistry, 57 Brahman heifers with 309.1 ± 3.04 kg BW 1,010 8.51 days age, were divided into three uniform groups assigned for 102 to treatments under a completely randomized design: 1) grazing forage only (F); 2) F+CaLCFA,...

To explore the association of the Newcastle disease virus (NDV) fusion (F) protein with cholesterol-rich membrane domains, its localization in detergent-resistant membranes (DRMs) in transfected cells was characterized. After solubilization of cells expressing the F protein with 1% Triton X-100 at 4 degrees C, ca. 40% of total, cell-associated F protein fractionated with classical DRMs with den...

Abstract BACKGROUND Altered expression of actin subunits is found in multiple cancers. Alpha-cardiac (ACTC1) expressed sonic hedgehog medulloblastoma (SHH MB) and regulates both cell survival migration. We hypothesized that cardiac has a unique structural functional role SHH MB may be post-natal granule progenitors (GCPs) which share similar gene profiles. METHODS Actin subunit protein (ACTC1, ...