A plasma protein required for the support of ristocetininduced platelet aggregation was isolated from antihemophilic factor concentrate and radiolabeled with 1251. A double-antibody radioimmunoassay was developed, with use of specific rabbit anti-Vill related antigen serum and goat anti-rabbit globulin. The assay is sensitive, reproducible, and technically simple to perform. Values obtained in ...

In classic von Willebrand's disease (vWd), assignment of the heterozygous genotype for genetic studies and diagnosis for clinical purposes (which are not exactly the same) are formidable problems. We have pointed out in the first report in this series that almost 50% of the members of two large kindred who transmitted this disease, and were therefore heterozygous, were scored as normal by the u...

Factor VIII-related antigen is a recognised marker of endothelial cells. A brief immunocytochemical study of its distribution in follicular carcinoma of the thyroid and its value in the recognition of vascular invasion by this tumour has been carried out. Ten cases of follicular carcinoma of the thyroid were studied. In each strong endothelial staining was found in the majority of vessels in th...

Three cases of epididymal adenomatoid tumor are presented. The adenoid compositions of the tumors lined by epithelial cells showed a canalicular pattern with large vascular spaces, tubular pattern with glandlike regions or plexiform pattern with connective tissue strands. Immunohistochemistry demonstrated positive cytoplasmic staining for keratin, but negative for carcinoembryonic antigen and f...

We recently described tolerance induction with factor VIII/IX, cyclophosphamide, and high-dose intravenous IgG in hemophilia A or B patients with coagulation inhibitory antibodies. Circulating noninhibitory antibodies complexed with factor IX have been demonstrated in tolerant hemophilia B patients. Similar findings are now described in six tolerant hemophilia A patients. Complexes between fact...

PURPOSE Kaposiform-hemangioendothelioma (KHE) is a rare vascular tumor,developing predominantly in infants and children. Despite its benign histology, this tumor frequently behaves aggressively,causing significant morbidity and mortality. The striking resemblance of its spindle cell proliferation to that seen in Kaposi Sarcoma (KS) led to the designation of KHE for this rare lesion. This work e...

In order to determine whether the glial neoplastic cells within the brain gliomas show human von Willebrand factor (factor VIII-related antigen [FVIII-rA]) expression, and the possible distribution of FVIII-rA positive glial cells within the neoplasm, morphological examination and computerised morphometric analysis were conducted in 35 brain neoplasms, divided into 2 groups: glioblastoma multif...