INTRODUCTION The aim of this study was to investigate the prevalence, phenotype, and genotype of Enterococcus faecalis isolated from saliva and root canals in patients with endodontic treatment failure. METHODS Samples were collected from 32 adults undergoing retreatment for periapical lesions after endodontic treatment performed at least 2 years previously. Isolates that were presumed to be ...

The prevalence of Enterococcus faecalis was investigated in diseased swine in Hunan Province, China between February 2007 and February 2011. A total of 385 diseased swine dissected in prosectorium from 10 representative administrative regions in Hunan Province were examined for the presence of E. faecalis. Forty-two out of 385 (10.91%) diseased swine were found to be infected with E. faecalis b...

Quorum-sensing systems control major virulence determinants in Enterococcusfaecalis, which causes nosocomial infections. The E. faecalis quorum-sensing systems include several virulence factors that are regulated by the cytolysin operon, which encodes the cytolysin toxin. In addition, the E. faecalis Fsr regulator system controls the expression of gelatinase, serine protease, and enterocin O16....

In vitro susceptibilities of 4,208 enterococci (83% Enterococcus faecalis isolates, 13.6% Enterococcus faecium isolates, and 3.4% isolates of other species) from patients in 27 European countries towards 16 antibiotics were determined. High-level resistance to gentamicin varied by country (range, 1 to 49%; mean, 22.6% +/- 12. 3%) and per species (19.7% E. faecalis isolates, 13.6% E. faecium iso...

Enterococcus faecalis is part of the commensal microbiota of humans and its main habitat is the gastrointestinal tract. Although harmless in healthy individuals, E. faecalis has emerged as a major cause of nosocomial infections. In order to better understand the transformation of a harmless commensal into a life-threatening pathogen, we developed a Recombination-based In VivoExpression Technolo...

introduction : the aim of this in vitro study was to evaluate the antimicrobial activity of four intracanal medicaments on enterococcus faecalis (e. faecalis). methods and materials: fifty extracted single-rooted human teeth were prepared with standard method. after contaminating the canals with e. faecalis , the samples were divided into one control and four experimental groups ( n =10). the t...

Enterococcus faecalis is becoming an increasingly important opportunistic pathogen worldwide, especially because it can cause life-threatening nosocomial infections. Treating E. faecalis infections has become increasingly difficult because of the prevalence of multidrug-resistant E. faecalis strains. Because bacteriophages show specificity for their bacterial hosts, there has been a growth in i...

Clustered, Regularly Interspaced Short Palindromic Repeats and their associated Cas proteins (CRISPR-Cas) provide prokaryotes with a mechanism for defense against mobile genetic elements (MGEs). A CRISPR locus is a molecular memory of MGE encounters. It contains an array of short sequences, called spacers, that generally have sequence identity to MGEs. Three different CRISPR loci have been iden...

INTRODUCTION The purpose of this study was to compare the antimicrobial efficacy of sodium hypochlorite (SH) and calcium hypochlorite (CH) against Enterococcus faecalis (E. faecalis) using quantitative real-time polymerase chain reaction (qPCR) analysis and also to compare their cytocompatibility on L929 murine fibroblasts using Mossman's tetrazolium toxicity (MTT) assay. METHODS AND MATERIAL...

Ampicillin-ceftriaxone combination therapy has become a predominant treatment for serious Enterococcus faecalis infections, such as endocarditis. Unfortunately, ceftriaxone use is associated with future vancomycin-resistant enterococcus colonization. We evaluated E. faecalis in an in vitro pharmacodynamic model against simulated human concentration-time profiles of ampicillin plus ceftaroline, ...