The binding interactions between ligands and receptors play a vital role in their biological functions. In this work, a label-free indirect competitive binding assay based on surface plasmon resonance (SPR) detectionwas developed, using fatty acid–human serumalbumin (HSA) as amodel system. In the assay, a fatty acid analog was immobilized on the surface of an SPR gold chip by self-assembly, and...

To understand the mechanism of long-chain fatty acid permeation of the plasma membrane in mammalian cells, the effects of changes in the cytoplasmic pH on the internalization of physiologically relevant, submicromolar concentrations of uncomplexed long-chain fatty acids were investigated in 3T3-L1 adipocytes. The acidification of the cytoplasm upon NH4Cl prepulsing of intact cells was accompani...

opium poppy (papaver somniferum l.) has two major products: alkaloids in the capsules and the seeds.  the seed contains oil, protein, carbohydrate, moisture and mineral matters. the seed oil is rich in unsaturated fatty acids, particularly linoleic and oleic acid. remaining meals after oil extraction are the important source for animal diets. the united nations recognize turkey and india as tra...

Fatty acid binding protein has been found in rat aortic endothelial cell membrane. It has been identified to be a 40-kDa protein that corresponds to a 40-kDa fatty acid binding protein with high affinity for a variety of long chain fatty acids isolated from rat heart myocytes. It is proposed that this endothelial membrane fatty acid binding protein might mediate the myocardial uptake of fatty a...

In order to determine anti-adipogenic effect, this study investigated 1β-hydroxy-2-oxopomolic acid (HOA) isolated from Agrimonia pilosa inhibits adipocyte differentiation and expression of adipogenic marker genes, such as peroxisome proliferator activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPα), glucose transporter 4 (GLUT4), adiponectin, adipocyte fatty acid-binding prote...

Angiopoietin-like protein 4 (ANGPTL4) is a potential circulating mediator connecting nutritional factors and fat metabolism, however, information lacking on the exact role of ANGPTL4 triglyceride metabolism in thigh muscles livers broilers. The objective this study was to determine changes broilers injected intravenously with ANGPTL4. In experiment 1, 36 male Arbor Acres at 35 days age were ran...