Concentrations of thermotolerant coliform bacteria, presumptive E. coll, and presumptive fecal streptococci were determined from ditches, brooks, and natural ponds in six agricultural areas and 22 uninhabited pristine areas in southern Finland in the summer of 1987. For comparison, the same fecal indicators were enumerated from the effluents of three wastewater t eatment plants. The objective w...

The aim of this article was to define the sampling level and method combination that captures antibiotic resistance at pig herd level utilizing qPCR antibiotic resistance gene quantification and culture-based quantification of antibiotic resistant coliform indicator bacteria. Fourteen qPCR assays for commonly detected antibiotic resistance genes were developed, and used to quantify antibiotic r...

Stormwater runoff can transport a variety of pollutants including heavy metals, excess nutrients, sediments, trash, etc. Several studies dating back to the 1970s have shown that a number of human pathogens are often present in stormwater and can lead to serious health risks. The resulting health and economic impacts can be significant since the presence of elevated concentrations of microorgani...

Anaerobic incubation of membrane filter cultures significantly enhanced detection of fecal coliforms in surface-water samples from recreational beaches. In contrast to standard aerobic incubation, anaerobic incubation suppressed overgrowth of masking, noncoliform bacteria but did not increase the frequency of fecal coliform recovery.

Fecal coliform concentrations increased significantly (F test) in the immediate vicinity of a maintenance dredging operation in the Mississippi River navigation channel. Increased counts were attributed to the disturbance and relocation of bottom sediments by dredging and a concomitant release of sediment-bound fecal coliforms.

Agricultural practices such as cattle grazing and animal manure application can contribute to relatively high runoff concentrations of fecal coliform (FC) and fecal streptococcus (FS). Available information, however, is inconsistent with respect to the effects of such practices as well as to measures that can discriminate among candidate sources of FC and FS. The objective of this study was to ...

due to uncontrolled dump of dried municipal sludge and its use by farmers as soil conditioner and/or fertilizer, an extensive research was conducted in order to determine the microbiological characteristics of municipal sludges produced at three major cities, namely, qateef, dammam and khobar in the eastern province of saudi arabia. sludge samples were collected, from sand drying beds, twice a ...

Total coliform (TC), fecal coliform (FC), and fecal streptococcus (FS) recoveries in chlorinated secondary sewage effluents were investigated by the membrane filter (MF) and multiple-tube (most probable number, MPN) methods. The LES two-step MF method was found to be comparable to the MPN procedure for determining TC. The TC detection was 1.5 times greater by the LES two-step technique than tha...