This study has revealed that a Clostridium perfringens ferredoxin gene (per-fdx) possesses a novel type of DNA curvature, which is formed by five phased A-tracts extending from upstream to downstream of the -35 region. The three A-tracts upstream of the promoter and the two within the promoter are located at the positions corresponding to A-tracts present in a C. perfringens phospholipase C gen...

Valentine, R. C. (University of Illinois, Urbana) and R. S. Wolfe. Role of ferredoxin in the metabolism of molecular hydrogen. J. Bacteriol. 85:1114-1120. 1963.-The metabolism of molecular hydrogen by Clostridium pasteurianum, Micrococcus lactilyticus (Veillonella alcalescens), and several other anaerobic bacteria was studied. Oxidation of hydrogen, using several electron-accepting substrates i...

Inorganic ammonium ions are assimilated by a coupled reaction of glutamine synthetase and glutamate synthase (GOGAT). In rice, three genes encoding either ferredoxin (Fd)-GOGAT, NADH-GOGAT1, or NADH-GOGAT2, have been identified. OsNADH-GOGAT2, a newly identified gene, was expressed mainly in fully expanded leaf blades and leaf sheaths. Although the distinct expression profile to OsNADH-GOGAT1, ...

The interaction between spinach ferredoxin and ferredoxin:NADP+ reductase was studied by varying pH and oxidation-reduction state. The Kd of the oxidized ferredoxin . ferredoxin:NADP+ reductase complex increases with increasing pH in the range from pH 6 to pH 8; the Kd is pH-independent above pH 9. These data are interpreted as showing 1 proton binding/complex at neutral pH. The extent of assoc...

To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in irondepleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased ...

Lovcnberg and Williams (1‘1 have reported that, when a solution of Clostridium pastewianum ferredoxin in 90% ammonium sulfate is treated on a column of DEAE-cellulose, ferredoxin is retained by the column. They showed t,hat, ferredoxin is eluted when the ammoniuni sulfate concentration is lowered to about 35c/, saturation. Thcsc observations suggested the method described in this paper for puri...

The flavin analogue 5-deazariboflavin is a convenient catalyst for the photoreduction of low-potential redox compounds. In an anaerobic medium with Tricine buffer as the electron donor, 5-deazariboflavin is capable of photo-reducing both ferredoxin and methyl viologen. We have used this method to conduct a comparative study of the Photosystem I photophosphorylation activities supported by the r...

Fhit protein is lost in most cancers, its restoration suppresses tumorigenicity, and virus-mediated FHIT gene therapy induces apoptosis and suppresses tumors in preclinical models. We have used protein cross-linking and proteomics methods to characterize a Fhit protein complex involved in triggering Fhit-mediated apoptosis. The complex includes Hsp60 and Hsp10 that mediate Fhit stability and ma...

A combination of structural, thermodynamic, and transient kinetic data on wild-type and mutant Anabaena vegetative cell ferredoxins has been used to investigate the nature of the protein-protein interactions leading to electron transfer from reduced ferredoxin to oxidized ferredoxin:NADP+ reductase (FNR). We have determined the reduction potentials of wild-type vegetative ferredoxin, heterocyst...

Magnetic susceptibilities of both reduced and oxidized ferredoxin from Clostridium pasteurianum were obtained in solution. Whereas the reduced form exhibits a Curie law behavior, the magnetic susceptibility of oxidized ferredoxin in fact increases with temperature and suggests extensive antiferromagnetic exchange coupling between the component iron atoms. Contact-shifted resonances are observed...