We identified a variant of the first bovine polyomavirus (BPyV1; family Polyomaviridae) in a lymph node of a Salers cow. As the 2 previously published genome sequences of this virus originated from fetal bovine serum and ground beef, respectively, this is the first BPyV1 genome that could be traced back to an individual.

Detailed Methods Cell Culture The human THP-1 macrophage-like cell line was obtained from American Type Culture Collection (ATCC, Manassas, VA). For experiments, cells were cultured at 4.0x10 cells/35-mm plate (Falcon Scientific, BD Biosciences) in RPMI 1640 supplemented with 10% fetal bovine serum (Sigma), β-mercaptoethanol (5x10 mol/L), 100 units/mL penicillin, and 100 μg/ml streptomycin and ...

This study was undertaken to examine the cell cycle characteristics of bovine fetal and adult somatic cells (fetal fibroblasts, adult skin and muscle cells, and cumulus cells) after culture under a variety of conditions; 1) growth to 60-70% confluency (cycling), 2) serum starvation, 3) culture to confluency. Cell -cycle phases were determined by flow cytometry with propidium iodide staining ena...

Tracking cellular 57Co-labelled cobalamin (57Co-Cbl) uptake is a well-established method for studying Cbl homeostasis. Previous studies established that bovine serum is not generally permissive for cellular Cbl uptake when used as a supplement in cell culture medium, whereas supplementation with human serum promotes cellular Cbl uptake. The underlying reasons for these differences are not fully...

BACKGROUND Mesenchymal stem cells are promising candidates in regenerative cell therapy. Conventional culture methods involve the use of animal substances, specifically fetal bovine serum as growth supplement. Since the use of animal-derived products is undesirable for human applications, platelet lysates produced from human platelets are an attractive alternative. This is especially true if pl...

We examined androgen modulation of proliferation of clonally derived AXC/SSh rat prostate cancer cells. C-family cells were maintained on medium without addition of androgens. D-family cells were maintained on medium containing 10(-7) M 5 alpha-dihydrotestosterone and T-family cells were maintained on medium containing 10(-7) M testosterone. Proliferation of all AXC/SSh prostate cancer cell lin...

Spermatozoa of the freeze-tolerant wood frog (Rana sylvatica) were used to develop a general protocol for the frozen storage of amphibian spermatozoa. Tolerance of spermatozoa to cryoprotective agents and freezing in suspension (-80 degrees C) was determined from rates of sperm lysis and dual-fluorochrome vital dye assays. We tested the efficacy of four cryoprotectants (Me2SO, methanol, glycero...