In recent years, microspectrophotometry of the Feulgen stain (1) has been extensively used as a method for determining amounts of desoxyribosenucleic acid (DNA) in individual cell nuclei (2-4). In spite of certain obvious advantages of this procedure which permits the DNA analysis of a single nucleus in situ directly under the microscope and its applicability to the study of the significance of...

A potential biomarker for early diagnosis of cancer is assessment of high nuclear DNA content. Conventional hematoxylin staining is neither stoichiometric nor reproducible. Although feulgen stain is stoichiometric, it is time consuming and destroys nuclear morphology. We used acidic thionin stain, which can be stoichiometric and also preserve the nuclear morphology used in conventional cytology...

To classify cases of non-Hodgkin's lymphoma in terms of expected clinical behaviour and survival, kinetic parameters measured by cytophotometry were assessed in 62 patients between 1978 and 1987. The influence of the number of cells with increased DNA content (more than 2N) on survival was evaluated. Analyses were carried out on the small samples obtained by needle aspiration biopsy of lymph no...

Studies correlating the production of infectious adenovirus (types 5 and 7) and the progression of the stages of virus-induced cytologic change in HeLa cells are presented. The results reveal a close relationship between the development of the characteristic nuclear changes and adenovirus synthesis. They suggest that cells manifesting the first stages of nuclear change, characterized by the app...

A method is presented in which a complex metal-ion (ammoniacal silver) is substituted for leuco-basic fuchsin in the well-known Schiff reaction for aldehydes. The reagent is reduced to metallic silver by tissue aldehydes and submicroscopic particles of the metal are deposited at the reactive sites. When the reagent is used in the Feulgen reaction chromosomes may be 'stained' in a specific manne...

The amount of DNA in individual megakaryocytes (MK) was measured by Feulgen photometry performed on smears of steady-state rat bone marrow. The MK were identified by acetylcholinesterase (ACHE) staining. The method described is particularly suitable in the study of immature MK, hitherto difficult to examine for DNA content. The main MK ploidies were 8N, 16N, and 32N, comprising 33.4% +/- 1.4%, ...

Cytophotometric DNA determination was performed on 8 glioblastomas, 4 astrocytomas, 2 oligoden drogliomas, and 4 meningiomas. Tumor cells were separated from a paraffin section and stained for DNA using the acriflavine-Feulgen nuclear reaction. Nonspecific dye-binding was blocked by azocarmin G staining. All the specimens were postirradiated to eliminate primary fluorescence and to stabilize th...