Tissue necrosis may be detected by the appearance of intracellular enzymes in extracellular spaces (Kibe & Nilsson, 1967) and this principle has been applied to ischaemic and hypoxic cardiac necrosis in man and animals (Roe & Starmer, 1975; Shell et al., 1973). Beating myocardial cells in culture represent an extremely reproducible preparation for studies of the deleterious effect of hypoxia on...

Streptokinase produces it-s lytic effect by activation of a profibrinolysin usually called plasminogen. Many attempt-s have been made to isolate this latter material for investigation without consistent success.#{176}’ ‘#{176} A new method for preparation of this material (plasminogen)’t ‘ 12 has recently been reported. In vivo stu lies of activated plasminogen (plasmin) in dogs and rabhit-&3 h...

“From 1946-1949, J.B. Duguid published his observations on the incorporation of fibrin into the walls of coronary arteries, on which he based his revival of Rokitansky’s thrombogenic theory of atherosclerosis.1 By the time I came to work in Duguid’s department at Newcastle the theory had created considerable interest among those working on blood coagulation, and he himself was writing that ‘in ...

IN THE COURSE of assaying the potency of a variety of fibrinolytic enzyme preparations in dogs and monkeys with isotopelabeled fibrin clots, we observed that the fibrinolytie activity disappeared rapidly from the systemic circulation. Dissolution of blood clots was noted to occur long after the disappearance of measurable circulating fibrinolytic activity. These experimental observations were c...