This study was aimed at evaluating the cloning and expression of three rumen microbial fibrolytic enzyme genes in a strain of Lactobacillus reuteri and investigating the probiotic characteristics of these genetically modified lactobacilli. The Neocallimastix patriciarum xylanase gene xynCDBFV, the Fibrobacter succinogenes beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase [EC 3.2.1.73]) g...

The addition of exogenous fibrolytic enzymes (EFEs) and length storage can affect the quality maize silage. Therefore, objective this study was to evaluate fermentative profile nutritive value silage treated with different doses EFEs ensiled for 30, 60, or 90 days. designed as completely randomized in a split-plot arrangement treatments, where four were assigned main plot three lengths sub-plot...

Abstract Purpose The present experiment aimed to evaluate date palm leaves (DPL) treated without or with fibrolytic enzymes as a feed for ruminants. Methods employed an in vitro wireless gas production system the dietary inclusion of DPL sun-dried, ensiled 45 days. different forms replaced berseem hay (300 g/kg diet) at 0, 25, 50, 75 and 100% diet. Results Dried linearly decreased asymptotic to...

This study aimed to evaluate the effects of cellulase and xylanase plus enzymes on in vitro ruminal fermentation of Eragrostis curvura hay, maize stover and a total mixed ration (TMR) at six levels of application. The feed samples were incubated for 2, 12, 24, 32, 48, 72, h in an in vitro batch culture with buffer and ruminal fluid, and the fibrolytic enzymes associated with the six levels of a...