Since the development of the polymerase chain reaction (PCR), many strategies have been designed to amplify one or more specific DNA sequences with a single set of oligonucleotide primers. Multiplex PCR is a strategy to amplify simultaneously either two or multiple DNA targets sequences in a single reaction (4). A variety of reaction conditions must be used to optimize multiplex-PCR strategies....

Transportation costs are a significant part of capital and operating costs of large open pit mines. Cost efficiency and high reliability of in pit crushing and conveying systems compared to conventional truck-shovel system makes it more appealing to be utilized in modern mining activities. In this paper the suitable location of an in pit crusher is investigated as a single hub location problem....

Hepatic pit cells are a population of large granular lymphocytes that substantially contribute to hepatic immunity. Studies have proven that pit cells have a role in liver regeneration, but the details of the relationship between pit cells and liver regeneration is not clear at present. We subjected rats to a two-third hepatectomy; pit cells with high purity were obtained with Percoll density c...

Pit-1 is a pituitary-specific transcription factor responsible for activating growth hormone (GH) and prolactin genes. Here, we describe the isolation of a rainbow trout cDNA clone that contains the entire Pit-1 coding region. The deduced amino acid sequence contains 358 residues, encoding a 39-kDa protein. Comparison of the protein sequences of the rainbow trout and rat Pit-1 shows that the 16...

Pit latrines can provide improved household sanitation, but without effective and inexpensive emptying options, they are often abandoned once full and may pose a public health threat. Emptying techniques can be difficult, as the sludge contents of each pit latrine are different. The design of effective emptying techniques (e.g., pumps) is limited by a lack of data characterizing typical in situ...

The hydraulic resistance of pit membranes was measured directly in earlywood vessels of Fraxinus americana and Ulmus americana. The area-specific resistance of pit membranes (r(mem)) was higher than modeled or measured values obtained previously for hardwood species, with r(mem) of 5.24 × 10(3) MPa·s·m(-1) for Fraxinus and 2.56 × 10(3) MPa·s·m(-1) for Ulmus. The calculated resistance of pit can...

BACKGROUND A pit latrine is the most basic form of improved sanitation which is currently used by a number of people around the globe. In spite of the wide spread use, known successes and advantages associated with pit latrines, they have received little attention in form of research and development. This review focuses on the usage and performance (filling, smell and insect nuisance) of pit la...

The POU transcription factor Pit-1 activates members of the prolactin/growth hormone gene family in specific endocrine cell types of the pituitary gland. Although Pit-1 is structurally conserved among vertebrate species, evolutionary changes in the pattern of Pit-1 RNA splicing have led to a notable "contraction" of the transactivation domain in the mammalian lineage, relative to Pit-1 in salmo...

We present recent diboson production measurements from the D0 experiment at Fermilab's Teva-tron collider. The production of ZZ was observed using leptonic final states. Zγ → ννγ was observed and used to set the most stringent limits from a hadron collider on anomalous Zγγ and ZZγ trilinear gauge couplings (TGCs). WW events with leptonic final states and WW +W Z events with semi-leptonic final ...

Macular detachment causes visual deterioration in 25-75% of patients with congenital optic disc pit. A number of treatment options have been reported to manage the macular detachment in optic pit. An optic disc pit represents a defect in the lamina cribrosa; theoretically, an ideal procedure to treat optic pit associated macular detachment would be one that prevents the flow of fluid across the...