Nonstationary metabolic flux analysis (NMFA) is at present a very computationally intensive exercise, especially for large reaction networks. We applied elementary metabolite unit (EMU) theory to NMFA, dramatically reducing computational difficulty. We also introduced block decoupling, a new method that systematically and comprehensively divides EMU systems of equations into smaller subproblems...

Theory and experience in metabolic engineering both show that metabolism operates at the network level. In plants, this complexity is compounded by a high degree of compartmentation and the synthesis of a very wide array of secondary metabolic products. A further challenge to understanding and predicting plant metabolic function is posed by our ignorance about the structure of metabolic network...

This work presents a new network centric architecture for multiple frame assignment (MFA) tracking. The architecture improves on earlier network tracking schemes by allowing trackers to broadcast decisions about their local soft-level associations, via the Soft Associated Measurement Reports (SAMRs). The SAMR may be followed by an “Oops” message, if the soft association was incorrect and must b...

A need has existed for the accurate identification of monofluoroacetate (MFA) poisoning in southern Africa. The development of a new method for the determination of MFA has made the analysis of a variety of biological samples (n = 50) feasible. The method has been used in the laboratory over 24 months. Monofluoroacetate was present in 66% of samples from cases of suspected poisoning, reflecting...

A cross-sectional study was carried out in 2005 and 2006 in three geographical areas of Madagascar to investigate and differentiate swine farm management and biosecurity practices in smallholder farming communities. Questionnaire data from a total of 709 pig farms were analysed using multiple factor analysis (MFA) and hierarchical cluster analysis (HCA). Variables describing management and bios...

The main requirement for metabolic flux analysis (MFA) is that the cells are in a pseudo-steady state, that there is no accumulation or depletion of intracellular metabolites. In the past, the applications of MFA were limited to the analysis of continuous cultures. This contribution introduces the concept of dynamic MFA and extends MFA so that it is applicable to transient cultures. Time series...

Abstract—We report in this paper the evolution of a physically based drop size distribution coupling the Maximum Entropy Formalism and the Monte Carlo method to solve the coagulation equation. Using the discrete or continuous population balance equation, the Mass Flow Algorithm is formulated taking into account interactions between droplets via coalescence. After proposing a kernel for coalesce...

This study focuses on the possible impact of the MFA phase-out on the world economy. Starting from analyzing trends in world textile and apparel trade (T&A), the study found that the developing countries were a growing factor in world T&A trade in recent decades. Moreover, using trade data from 91 countries over 37 years, the empirically investigate of the study indicates a strong positive rela...

Material flows and their contributions to fuel properties are balanced for the mechanical section of a mechanical-biological treatment (MBT) plant producing solid recovered fuel (SRF) for the UK market. Insights for this and similar plants were secured through a program of sampling, manual sorting, statistics, analytical property determination, and material flow analysis (MFA) with error propag...