Method We report a 50 year old patient, who works as a cook. A year ago she presented two episodes of anaphylaxis after eating undercooked fresh fish. Thereafter she removed raw fish from her diet. Ten months later after handling fish (eviscerating it), an hour after hand injuring, she presented itching hands and head, epigastric pain, nausea and severe generalized urticaria. Attended the emerg...

AIM To compare the total and organic mercury content in sea-fish samples from Croatia, where fishing occurs in a closed part of the Mediterranean Sea, and from other countries fishing mostly in the oceans. METHOD During 1997, we collected at the Zagreb fish market a total of 115 samples of fifteen kinds of fish including bathypelagic, pelagic and elasmobranch fish, caught in the wider coastal...

We have developed a fast, simple, and accurate DNA-based screening method to identify the fish species present in fresh and processed seafood samples. This versatile method employs PCR amplification of genomic DNA extracted from fish samples, followed by restriction fragment length polymorphism (RFLP) analysis to generate fragment patterns that can be resolved on the Agilent 2100 Bioanalyzer an...

Introduction The question of bacterial culture sensitivity for individual versus pooled samples was discussed at the 2001 Fish Health Biologist Meeting in Tucson, AZ, as procedures for the USFWS Handbook were being developed. The USFWS Handbook strives to conform with methods specified by the Office International des Epizooties (OIE 2000) and the American Fisheries Society (AFS) Blue Book (Thoe...

Direct numerical simulation of self-propelled swimming of three dimensional bionic fish and fish school in a viscous flow in a tank are investigated. The swimming rule and the profile of 3D fish from previous experimental measurements of living fish are used in the study. A parallel software package for the 3D moving boundary problem is obtained, which combines the adaptive multi-grid finite vo...

Fishborne trematode (FBT) metacercariae were investigated in fish from 3 Provinces of Lao PDR. Total 242 freshwater fish of 40 species were collected in local markets of Luang Prabang (59 fish of 16 species), Khammouane (81 fish of 19 species), and Saravane (97 fish of 14 species), and each of them was examined by artificial digestion method. Four species of metacercariae (Opisthorchis viverrin...

A method for the analysis of 10 perfluorinated compounds (PFCs) in whole fish homogenate is presented and applied to 60 fish samples collected from the Ohio, Missouri, and upper Mississippi Rivers in 2005. Method accuracy ranged between 86 and 125% with limits of quantitation between 0.2 and 10 ng/g wet weight. Intra- and inter-batch precision was generally +/-20%. Perfluorooctane sulfonate (PF...

Vesicular stomatitis virus glycoprotein G-pseudotyped mouse retroviral vectors have been used as mutagens for a large-scale insertional mutagenesis screen in the zebra fish. To reproducibly generate high-titer virus stocks, we devised a method for rapidly selecting cell lines that can yield high-titer viruses and isolated a producer cell line that yields virus at a high titer on zebra fish embr...