Flavodoxins are small flavoproteins (M, 15000-22000), that have been found in several strains of bacteria and algae [ I ] The protein contains one molecule of non-covalently bound FMN and hnctions biochemically as a one-electron carrier between the semiquinone and hlly reduced forms. Under iron-deficient conditions the protein is produced by Megasphaera elsdenii to replace the iron-sulphur prot...

Lys 10 10 8 4 8 7 5 12 9 13 9 Try 4 4 4 1 2 1 6 3 4 3 4 6 His 0 0 0 1 0 3 I 4 1 0 0 Arg 2 2 2 6 3 3 4 4 8 6 2 Asp 18 17 16 20 17 1 3 1 4 22 28 25 21 28 Thr 4 5 8 6 9 6 8 14 16 8 8 Ser 14 8 7 7 8 4 4 11 11 15 12 Glu 19 21 18 17 18 1 6 1 7 25 22 16 23 26 Pro 4 4 5 4 6 3 4 5 7 5 3 Gly 17 14 14 19 14 11 19 25 28 21 19 Ala 14 6 18 18 15 11 15 24 20 15 12 CvS 1 3 2 5 5 3 2 5 2 1 1 Val 15 10 13 10 16 ...

The Escherichia coli sulfur starvation utilization (ssu) operon includes a two-component monooxygenase system consisting of a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent flavin mononucleotide (FMN) reductase, SsuE, and a monooxygenase, SsuD. SsuE is part of the flavodoxin-like superfamily, and we report here the crystal structures of its apo, FMN-bound, and FMNH2-bound forms a...

Ribonucleotide reductase (RNR) catalyzes the conversion of nucleotides to deoxynucleotides and is essential in all organisms. Class I RNRs consist of two homodimeric subunits: alpha2 and beta2. The alpha subunit contains the site of nucleotide reduction, and the beta subunit contains the essential diferric-tyrosyl radical (Y*) cofactor. Escherichia coli contains genes encoding two class I RNRs ...

BlsE, a predicted radical S-adenosyl-L-methionine (SAM) protein, was anaerobically purified and reconstituted in vitro to study its function in the blasticidin S biosynthetic pathway. The putative role of BlsE was elucidated based on bioinformatics analysis, genetic inactivation and biochemical characterization. Biochemical results showed that BlsE is a SAM-dependent radical enzyme that utilize...