apoptosis could be a major mechanism of antitumor effect of tamoxifen. therefore this study is designed to characterize the kinetic behavior of tamoxifen-induced apoptosis in the estrogen receptor positive (er+) and negative (er-) cell lines, mcf-7 and mda-mb-468. frequency of cell death was examined by trypan blue and acridine orange staining. annexin v-fluorescein/pi was used in flow cytometr...

adverse effects  following immunization  to  vaccines (aefi)  are considered  extremely rare events, the occurrence of which could gain a major role in optimizing allergy diagnosis by cellular tests. the urgent need to eradicate infectious diseases from population, is the main goal of vaccination campaign, therefore its successful outcome should be almost undisputable. basophil activation test ...

background: acute promyelocytic leukemia (apl) is a subclass of acute myeloid leukemia. the chromosomal aberration in 95% of apl cases is t(15; 17) (q22; q21), which prevents cell differentiation. characterization of the underlying molecular lesion is valuable in determining optimal treatment strategy. the goal of this study was to develop a new and powerful flow- fish technique to detect the l...

results in total, 261 patients (119 sarcoidosis and 142 non-sarcoidosis ilds) were enrolled. the median (interquartile range) bal cd4/cd8 ratio and lymphocyte fraction were significantly higher in sarcoidosis than in non-sarcoidosis ilds: 3.88 (3.76) versus 0.88 (1.01), respectively, and 20.6 (28.3) versus 6.0 (13.7), respectively. t cell receptor γ delta, cd16+56+, cd103+, cd8+103+, and cd3+16...

objective: to classify different types of acute leukemia based on cooperative game theory and shapley value. materials and methods: in this study, patients data were collected from flow cytometry tests of the iran blood transfusion organization (ibto) have been used. 304 different diagnosed samples in 8 classes of acute leukemia were investigated. samples were initially in numerical format. i...

Background: Quantification of fetal red blood cells (RBCs) in maternal blood is of great importance to calculate appropriate dose of post-deliver anti D immunoglobulin in a rhesus D (RhD)-negative woman. Objective: The aim of this study is to evaluate a direct immunofluorescence flow cytometry technique in artificial and clinical samples and compared it t...