This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample--A suspension of micro particles or blood cells--is injected into a sheath fluid streaming at a substantially ...

A simple one-step flow focusing method was used to embed both magnetic nanoparticles and quantum dots in microbeads in controlled ratios to generate a large library of molecular barcodes for biological applications.

Introduction Droplet-based microfluidics is a research area that has caught the attention of different disciplines in the past decade[1]. Droplets of immiscible fluids (typically water in oil W/O or oil in water O/W) have been used as confined pL-nL size reaction chambers in which reagents and conditions are precisely controlled. Applications of this technology include: single cell assays, gas-...

We have demonstrated a microfluidic device that can not only achieve three-dimensional flow focusing but also confine particles to the center stream along the channel. The device has a sample channel of smaller height and two sheath flow channels of greater height, merged into the downstream main channel where 3D focusing effects occur. We have demonstrated that both beads and cells in our devi...

In the focusing problem, we study solutions to the porous medium equation ∂tu = (u) whose initial distributions are positive in the exterior of a compact 2D region and zero inside. We assume that the initial interface is elongated (i.e., has an aspect ratio > 1), and possesses reflectional symmetry with respect to both the xand y-axes. We implement a numerical scheme that adapts the numerical g...

This paper reports a method to control the bubble size generated in a microfluidic flow-focusing configuration. With an ultrasonic transducer, we induce acoustic streaming using a forward moving, oscillating gas-liquid interface. The induced streaming substantially affects the formation process of gas bubbles. The oscillating interface acts as a pump that increases the gas flow rate significant...

and become focused. The focused unsaturated flow will increase relative permeability of its path, and cause flow A numerical modeling study is presented to investigate flow– further focusing. Focused flow paths exist in the unsatufocusing phenomena in a large-scale fracture network, constructed rated rock of the Yucca Mountain site. For example, using field data collected from the unsaturated z...

Detection and sorting of particles and cells in a continuous flow stream is of great importance to downstream biological studies and high-throughput screening. Many particle separation techniques require peripheral devices, complex channel designs or setups that can limit the process and its applications. Here, we present a smart-phone attachable continuous flow magnetic focusing device as an i...

In this work, we investigate the behavior of dilute and semidilute polymer solutions flowing in a microfluidic flow focusing device, in which an inlet stream is stretched by two balanced lateral streams. By varying the flow rates in the inlet and lateral channels and their ratio, several types of flow transitions are observed and the resulting velocity fields are analyzed both upstream and down...

Disposable, inexpensive microfluidic devices have the potential to become a robust tool for proteomic research for separating and identifying proteins and protein complexes. In this work, a preparative scale free flow IEF isoelectric focusing (FFIEF) device was designed, investigated, and optimized. A constant electric field of up to 350V/cm was generated across the separation channel upon opti...