We studied phospholipid topology and transbilayer mobility in red cells during blood storage. The distribution of phospholipids was determined by measuring the reactivity of phosphatidylethanolamine with fluorescamine and the degradation of phospholipids by phospholipase A2 and sphingomyelinase C. Phospholipid mobility was measured by determining transbilayer movements of spin-labeled phospholi...

Diaryl-furanones are specific analytical reagents for the biochemical detection of primary amines by fluorescence techniques. Well-known fluorescamine (Fluram) and 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF), yielding fluorescent products with λem at 480–490 nm. Although reaction claim to be pyrrolinones, recent studies show that they really 3-oxopyrrole (pyrrolone) derivatives. Both have been...

A novel, selective, and sensitive reversed phase high-performance liquid chromatography (HPLC) method coupled with fluorescence detection has been developed for the determination of tobramycin (TOB) in pure form, in ophthalmic solution and in spiked human plasma. Since TOB lacks UV absorbing chromophores and native fluorescence, pre-column derivatization of TOB was carried out using fluorescami...

Three selective, inexpensive and validated methods were developed and optimized for determination of antiepileptic drug, topiramate in drug substance and drug product as well as in the presence of its acid, base and thermal degradants. The first method is micelle-enhanced spectrofluorimetric assay based on the reaction between the studied drug and fluorescamine to give highly fluorescent deriva...