Single-molecule spectroscopy is an important new approach for studying the intrinsically heterogeneous process of protein folding. This Review illustrates how different single-molecule fluorescence techniques have improved our understanding of mechanistic aspects in protein folding, exemplified by a series of recent experiments on a small protein.

This paper describes the design of an active, integrated CMOS sensor array for fluorescence applications which enables time-gated, time-resolved fluorescence spectroscopy. The 64-by-64 array is sensitive to photon densities as low as 8.8 × 10(6) photons/cm(2) with 64-point averaging and, through a differential pixel design, has a measured impulse response of better than 800 ps. Applications inc...

Quantitative determination of the density and conformation of DNA molecules tethered to the surface can help optimize and understand DNA nanosensors and nanodevices, which use conformational or motional changes of surface-immobilized DNA for detection or actuation. We present an interferometric sensing platform that combines (i) dual-color fluorescence spectroscopy for precise axial co-localiza...

Data Exchange is a simple data model designed to interface, or `exchange', data among different instruments, and to enable sharing of data analysis tools. Data Exchange focuses on technique rather than instrument descriptions, and on provenance tracking of analysis steps and results. In this paper the successful application of the Data Exchange model to a variety of X-ray techniques, including ...

The main purposes of this study were to select a photochromic dye and to develop an active welding filter with a photochromic layer. A series of functionalized spirobenzopyranoindolins were synthesized and their photophysical and photochemical properties were investigated in a solution using absorption and emission spectroscopy. Time-resolved fluorescence spectroscopy measurements were used to ...

In copper(I) complex [Cu(dmphen)(2)]+ (dmphen = 2,9-dimethyl-1,10-phenanthroline), a "flattening" structural change is induced with 1MLCT excitation, which is a prototype of the structural change accompanied with Cu(I)/Cu(II) conversion in copper complexes. Femtosecond and picosecond emission dynamics of this complex were investigated in solution at room temperature with optically allowed S(2) ...

The [17.7]1-X(1)Sigma(+) (0,0) band of AuF at 566 nm has been studied by laser excitation spectroscopy. The molecule was prepared in a dc electric discharge by flowing a dilute mixture of SF(6) in argon through a hollow gold cathode. The rotational structure of the band has been analyzed for the first time, yielding accurate values for the rotational and Omega-type doubling constants of the upp...

We studied the time-resolved fluorescence of isolated RC-LH1 complexes from Rhodopseudomonas palustris as a function of the photon fluence and the repetition rate of the excitation laser. Both parameters were varied systematically over 3 orders of magnitude. On the basis of a microstate description we developed a quantitative model for RC-LH1 and obtained very good agreement between experiments...

5,15-bis(4-N-methylpyridyl)-10,20-diphenylporphyrin (5,15-DiMPyP, also known as t-H2Pagg) is capable of forming long-range ordered DNA-templated aggregates in solution. The present work reports the study of fluorescent properties of 5,15-DiMPyP, both monomeric and aggregated in presence of DNA, performed by means of steady-state and time-resolved fluorescence spectroscopy. The S1 state of free ...