The constraint-based approach to analysis of biochemical systems has emerged as a useful tool for rational metabolic engineering. Flux balance analysis (FBA) is based on the constraint of mass conservation; energy balance analysis (EBA) is based on non-equilibrium thermodynamics. The power of these approaches lies in the fact that the constraints are based on physical laws, and do not make use ...

In this article the metabolic flux analysis of growing CHO-320 cells is performed for a detailed metabolic network which involves 100 reactions and embraces all the significant pathways describing the metabolism of CHO cells. The purpose is to investigate the efficiency of the flux analysis when it is based on a relatively small set of extracellular measurements that can be easily achieved in m...

Metabolism is a complex process for energy production for cellular activity. It consists of a cascade of reactions that form a highly branched network in which the product of one reaction is the reactant of the next reaction. Metabolic pathways efficiently produce maximal amount of biomass while maintaining a steady-state behavior. The steady-state activity of such biochemical pathways necessar...

Understanding the system-level adaptive changes taking place in an organism in response to variations in the environment is a key issue of contemporary biology. Current modeling approaches, such as constraint-based flux-balance analysis, have proved highly successful in analyzing the capabilities of cellular metabolism, including its capacity to predict deletion phenotypes, the ability to calcu...

The identification of a set of genetic manipulations that result in a microbial strain with improved production capabilities of a metabolite with industrial interest is a big challenge in Metabolic Engineering. Evolutionary Algorithms and Simulated Annealing have been used in this task to identify sets of reaction deletions, towards the maximization of a desired objective function. To simulate ...

couplings are needed to explain the RD and RD∗ anomalies. We show that when the upper limit of BR(Bc → τ ν̄τ ) < 30% is included, RD can be significantly enhanced while RD∗ < 0.27. With the BR(Bc → τ ν̄τ ) constraint, we find that the τ -lepton polarizations can be still affected by the charged-Higgs effects, where the standard model (SM) predictions are obtained as: P τ D ≈ 0.324 and P τ D∗ ≈ −0...

Previous studies on emotion classification mainly focus on the writer's emotional state. By contrast, this research emphasizes emotion detection from the readers' perspective. The classification of documents into reader-emotion categories can be applied in several ways, and one of the applications is to retain only the documents that trigger desired emotions to enable users to retrieve document...

Flux balance analysis (FBA) is a linear programming-based framework widely used to predict the behavior, in terms of the resulting flux distribution, of cellular organisms in different media. FBA models are constructed using only stoichiometric information, and for this reason they sometimes fail in predicting fluxes precisely. In this work, we formally define the concept of hybrid FBA/kinetic ...

Accurate mapping of temporal changes in urban land use and land cover (LULC) is important for monitoring urban expansion and changes in LULC, urban planning, environmental management, and environmental modeling. In this study, we present a feature-based approach of the decision tree classification (FBA-DTC) method for mapping LULC based on spectral and topographic information. Landsat 5 TM and ...

MetaNetX.org is a website for accessing, analysing and manipulating genome-scale metabolic networks (GSMs) as well as biochemical pathways. It consistently integrates data from various public resources and makes the data accessible in a standardized format using a common namespace. Currently, it provides access to hundreds of GSMs and pathways that can be interactively compared (two or more), a...