The role of cysteine residues for structure and function of formaldehyde dehydrogenase from Pseudomonas putida was analysed by amino acid sequence comparison, homology-based structure modeling, site-directed mutagenesis, and chemical modification. Five out of seven cysteine residues found in the enzyme were concluded to coordinate with an active site zinc (Cys-46) and structural zinc atoms (Cys...

2-Hydroxybenzyl alcohol is an important active pharmaceutical ingredient for the production of many drugs, in particular gastrodin, which has a wide range beneficial effects on epilepsy, Alzheimer’s disease, Parkinson’s affective disorders, cerebral ischemia, cognitive disorders. It known that 2- and 4-hydroxybenzyl alcohols mixtures both compounds are obtained by interaction phenol with formal...

Methanol poisoning is a relatively rare but potentially serious medical emergency. Toxicity results when methanol is successively oxidized to the active metabolites formaldehyde and formic acid. We report a case of a 23-year-old male, a high daily alcohol consumer, who attended the local primary health care centre complaining of sudden visual loss. A presumed diagnosis of methanol intoxication ...

The dehalogenation in vivo of brominated aliphatic hydrocarbons such as methyl bromide (1) and bromochloromethane (2) has been demonstrated by recovery of inorganic bromide from blood and urine. The intracellular formation of bromide and methyl alcohol from methyl bromide has been advanced as an explanation for its prolonged toxic effect (3). Evidence for the splitting of chlorinated aliphatic ...

This article reports the cloning of the genes encoding the Arabidopsis and rice class III ADH enzymes, members of the alcohol dehydrogenase or medium chain reductase/dehydrogenase superfamily of proteins with glutathione-dependent formaldehyde dehydrogenase activity (GSH-FDH). Both genes contain eight introns in exactly the same positions, and these positions are conserved in plant ethanol-acti...

Background: Formaldehyde is one of the most serious air contaminant that can be found everywhere. Some of the source of formaldehyde include product of building materials, decorated materials, cigarette smoke, etc. Aldehydes compounds especially formaldehyde are used as disinfectants for sterilization in different industries. In this study we measured formaldehyde workplace exposure with NIOS...

Three colorimetric reagents for the determination of formaldehyde, the Nash reagent (ammonia plus acetylacetone), Purpald (4-amino-3-hydrazino-5-mercapto-1,2,4-triazole), and N-methylbenzothiazolinone-2-hydrazone (MBTH), were compared for the determination of methanol when used in conjunction with alcohol oxidase. The combination of alcohol oxidase plus the commonly used Nash reagent was specif...

Alcoholic liver disease has been associated with abnormalities in receptor-mediated endocytosis (RME) which results in abnormal degradation of metabolically altered proteins. Model systems using formaldehyde-modified albumin (f-Alb) have shown an impairment in RME following chronic alcohol consumption utilizing both in situ perfused rat livers and isolated rat liver endothelial cells (LECs). Th...

 Background: Human hair can be used as an inexpensive and accessible adsorbent to remove a variety of pollutants from air. Although several studies have been done on removal of formaldehyde from wastewater by human hair, to date no study has investigated using hair to remove formaldehyde from air. Therefore, the aim of this study was to remove formaldehyde from synthetic contaminated a...

-The present work is focused on the heterogeneous photocatalytic degradation of Amaranth dye over immobilized nano-crystals of TiO2. Immobilization has been an acceptable approach to overcome the drawbacks encountered with powder suspensions. Immobilization is performed using polyvinyl alcohol-formaldehyde binder. This method of immobilization does not require thermal heating at high temperatur...