Preparations of pyruvate formate-lyase were made from Escherichia coli cells. Net reversal of the "phosphoroclastic split" of pyruvate was readily demonstrated with these preparations. Incubation of acetyl phosphate with formate resulted in the accumulation of pyruvate in concentrations up to 0.5 mm. Catalytic amounts of coenzyme A were essential. Pyruvate was also readily formed from acetyl co...

Formate (l), serine (2)) formaldehyde (3), and methionine (4) each can serve as the donor of the carbon atom for the biosynthesis of the methyl group of DNA’-thymine. The utilization of formate for the biosynthesis of DNA in vitro has been reported previously (5-g), and in most instances the uptake of formate carbon under these conditions has been primarily into the methyl group of thymine (5)....

Anaerobic, but not aerobic, cultures of Escherichia coli accumulated Tc(VII) and reduced it to a black insoluble precipitate. Tc was the predominant element detected when the precipitate was analyzed by proton-induced X-ray emission. Electron microscopy in combination with energy-dispersive X-ray analysis showed that the site of Tc deposition was intracellular. It is proposed that Tc precipitat...

The pathway of the methane fermentation of formate was investigated in several organisms by Stephenson and Stickland (1933), Barker (1941) and Stadtman and Barker (1951). Since in several instances H2 and C02 were generated during fermentation, the decomposition of formate would probably involve some prior oxidation to C02 and subsequent reduction to CH4 by the C02-reduction scheme of Van Niel ...

Pendred syndrome, characterized by congenital sensorineural hearing loss and goiter, is one of the most common forms of syndromic deafness. The gene causing Pendred syndrome (PDS) encodes a protein designated pendrin, which is expressed in the thyroid, kidney, and fetal cochlea. Pendrin functions as an iodide and chloride transporter, but its role in the development of hearing loss and goiter i...

The considerations which prompted an investigation of the r61e of folic acid in formate metabolism were discussed in an earlier paper (1) in which it was reported that rats deficient in this vitamin incorporated less of a standard dose of CY4-formate into certain amino acids and heme than did rats fed folic acid. There is abundant microbiological evidence to indicate an interrelationship betwee...

Methyl formate is absorbed through the lungs (Schrenk et al., 1936). The death of a 19-month-old child after occlusive application of a methyl formate insecticide on the scalp for 20 min indicates a good penetration of methyl formate through the skin (Gettler, 1940). Acute intoxications were also reported in sick children after application of alcohol-soaked (methanol or ethanol) cloth on the ab...

Granules from a mesophilic upflow anaerobic sludge blanket reactor were disintegrated, and bacteria utilizing only hydrogen or formate or both hydrogen and formate were added to investigate the role of interspecies electron transfer during degradation of propionate and butyrate. The data indicate that the major electron transfer occurred via interspecies hydrogen transfer, while interspecies fo...