OBJECTIVE The aim of this study was to estimate the content of peptidoglycans in cutting fluids (CFs) and to assess the possibility of using them as a marker of bacterial contamination in this type of occupational environment. MATERIALS AND METHODS A total of 11 samples of CFs were collected: 8 were taken from the working machine systems and 3 were unused CF samples. The peptidoglycans were d...

In the United States, 1.2 million workers are exposed to metalworking fluids. During operations, aerosols are produced and airborne contaminants can be inhaled. Although biocides are used to control the bacterial content of metalworking fluids, they can create health-related problems, and their efficiency remains to be proved. The objectives of this project were (1) to verify whether rigorous c...

A nested PCR, developed for the detection of equine arteritis virus (EAV) in semen, detected less than 2.5 PFU of EAV per ml of naturally infected seminal plasma. Based on results from testing 88 semen samples from 70 stallions, the sensitivity and specificity of the test were 100 and 97%, respectively.

The aims of this study were to count and identify sucrose positive and negative vibrios isolated from cultivated Crassostrea rhizophorae oysters during their growing cycle. Every month for 12 months, 10 to 18 oysters were collected for study. Collections occurred at the Center for Studies of Coastal Aquaculture (CSCA), which is associated with the Institute of Marine Science, Labomar, located i...

Gardnerella vaginalis was isolated from 22 (38%) of 58 semen samples obtained from men attending an infertility clinic. Counts ranged from 1.2 X 10(3) to greater than 10(7) colony forming units (cfu)/ml. There was no association between the isolation of G vaginalis and the sperm count. Twenty (34.4%) samples contained non-sporing anaerobes and nine (15.5%) both anaerobes and G vaginalis. The in...

Melting phase relations of the diamond-forming olivine (Ol)–jadeite (Jd)–diopside (Di)–(Mg, Fe, Ca, Na)-carbonates (Carb)–(C-O-H-fluid) system are studied in experiments at 6.0 GPa polythermal Ol74Carb18.5(C-O-H)7.5-Omp74Carb18.5(C-O-H)7.5 section, where Ol = Fo80Fa20, Omp (omphacite) Jd62Di38 and Carb (MgCO3)25(FeCO3)25(CaCO3)25(Na2CO3)25. The peritectic reaction jadeite-bearing melts with for...

Cerebrospinal fluid (CSF) specimens from pediatric patients with meningitis were examined for their concentration of microbes and the relationship of this count to the bacteremia levels, microscopy results, and polymorphonuclear leukocyte concentration. A total of 2,031 consecutive CSF specimens were analyzed, of which 63 (3.1%) were positive by culture from the same number of patients. We obse...

Rabbits with experimentally induced pneumococcal meningitis were given single 25-mg/kg doses of apalcillin or cefpiramide. Mean percentages of the drug concentration in cerebrospinal fluid versus that in blood serum were 7.6% with apalcillin and 3.9% with cefpiramide. Bactericidal activity in cerebrospinal fluid resulted in mean reductions of from 4 to 5 log10 CFU/ml, and cerebrospinal fluid cu...