The study was carried out in Sahiwal, Holstein Friesian, Jersey and crossbred cattle and Murrah, Bhadwari, Jaffarabadi, Nagpuri and Surti buffaloes maintained at different organized herds to work out the polymorphism at growth hormone locus and study its effect on birth weight. A 223 bp fragment of the gene was amplified and digested with Alu I restriction enzyme. Two alleles, L and V with thre...

Approximately 20% of the beef tonnage produced in the United States is derived from Holsteins, through harvested dairy beef or cull dairy cows (Randy Blach, Cattle-Fax, personal communication). This represents a significant contribution to the U.S. beef supply. More and more large commercial feed yards have seen the opportunity available with feeding Holstein steers. Inherent variation between ...

Four cattle breeds (Gyr, Nellore, Guzerat and Holstein) were analyzed by amplification of genomic DNA using microsatellite loci to evaluate the genetic diversity within and among them. DNA samples of 18 animals from each breed were collected to access the genetic content of them. Allele frequencies were calculated and used to generate a Nei’s genetic distance matrix what was used to build a den...

The synaptonemal complex (SC) was analyzed in four F, hybrids of Bos taurus taurus and B. faurus zndicus including Gyr-Simmental (G-S), Nelore Simmental (N-S), Gyr-Holstein-Friesian (G-H) and Nelore-Piemontese (N-P). We analysed the frequency of various types of SC abnormalities and the frequency of cells with SC abnormalities. The results were compared with similar observations made on purebre...

Defi ciencies of Factor XI (FXID) and Uridine Monophosphate Synthase (DUMPS) are autosomal recessive inherited diseases in Holstein cattle breed. The aim of this study was to investigate whether FXI defi ciency and DUMPS alleles exist in Holstein cattle reared in Antalya region Turkey or not. 504 Holstein cows were used for this study. PCR (Polymerase Chain Reaction) was used for determination ...

The objective of this study was to identify twinning rate quantitative trait loci (QTL) by typing pooled samples in a preliminary screening followed by interval mapping to test QTL effects. Four elite North American Holstein half-sib sire families with high twinning rate predicted transmitting abilities (PTA) were used in this study. Chromosomes 5, 7, 19 and 23 were not genotyped as these chrom...

*Corresponding Author Rajesh. K. Patel [email protected] The present study investigated the occurrence of an autosomal recessive genetic disease, Bovine Citrullinaemia caused by mutation in Argininosuccinate Synthase (ASS) gene, in Indian Holstein cattle. The Polymerase chain reactionRestriction fragment length polymorphism (PCR-RFLP) analysis was performed on a group of 120 Holstein bulls to...

Recent reports identified DGAT1 (EC 2.3.1.20) harboring a lysine to alanine substitution (K232A) as a candidate gene with a strong effect on milk production traits. Our objective was to estimate the frequency of the DGAT1 K232A polymorphism in the main Zebu and Taurine breeds in Brazil as well as in Zebu x Taurine crossbreds as a potential QTL for marker-assisted selection. Samples of 331 anima...

Induction of twinning by ipsilateral nonsurgical transfer of two frozen-thawed Japanese Black bovine embryos to each of 20 Holstein and 26 Japanese Black cows, that had been kept under a stable in private farm conditions, was examined. The cows were monitored every 20 days from Day 2.5 to Day 65 of gestation for pregnancy and fetus survival (estrus is Day 0). Seventy-five per cent ( 1.5 of 20),...