Frameshift mutant trpA21 was isolated after ultraviolet treatment and frameshift mutant trpA540 after ICR191-A (an acridine derivative) treatment of wild-type Escherichia coli K-12. The A proteins of spontaneous and ICR191-A-induced partial revertants of these mutants contained altered amino acid sequences one residue shorter than the comparable sequence in the A protein of wild-type bacteria. ...

According to the prevailing model, frameshift-suppressing tRNAs with an extra nucleotide in the anticodon loop suppress +1 frameshift mutations by recognizing a four-base codon and promoting quadruplet translocation. We present three sets of experiments that suggest a general alternative to this model. First, base modification should actually block such a four-base interaction by two classical ...

The CRISPR/Cas9 prokaryotic adaptive immune system and its swift repurposing for genome editing enables modification of any prespecified genomic sequence with unprecedented accuracy and efficiency, including targeted gene repair. We used the CRISPR/Cas9 system for targeted repair of patient-specific point mutations in the Cytochrome b-245 heavy chain gene (CYBB), whose inactivation causes chron...

Since the identification of the breast and ovarian cancer susceptibility genes BRCA1 and BRCA2, a large number of different germline mutations in both genes have been found. 2 The BRCA1/2 germline mutations reported to date comprise a broad spectrum of sequence variants, mainly frameshift and nonsense mutations that result in truncated proteins. A minor proportion of the documented sequence var...

The frequency and specificity of mutations produced in uitro by eucaryotic DNA polymerase-@ have been determined in a forward mutation assay using a 250base target sequence in M13mp2 DNA. Homogeneous DNA polymerase-@, isolated from four different sources, produces mutations at a frequency of 4-6%/ single round of gap-filling DNA synthesis. DNA sequence analyses of 460 independent mutants res...