The Potential Usefulness of Free Fetal DNA for Prenatal Gender Determination in Blood Plasma Pregnant Goat

Fetal cells, specifically fetal erythroblasts, as well as cell-free fetal DNA are present in the maternal circulation. Both are currently being investigated as a means for the non-invasive risk-free analysis of fetal genetic traits. The origin of this cell-free fetal DNA in the maternal circulation is currently unclear. Since numerous fetal erythroblasts have been demonstrated to exhibit an apo...

Discovery of cell free fetal DNA in 1997 has deeply changed the outlook of prenatal diagnosis approaches as most of the clinically established screening tests are not sensitive/specific enough while the current practical diagnostic tests are also invasive in their nature. The most common prenatal screening test is routinely practiced for the diagnosis of Down syndrome (DS) which includes a 10% ...

background: the discovery of circulating fetal dna in maternal blood led to the discovery of new strategies to perform noninvasive testing for prenatal diagnosis. objective: the purpose of the study was to detect fetal aneuploidy at chromosomes 13, 18, 21, x, and y by analysis of fetal cell-free dna from maternal blood, without endangering pregnancy. materials and methods: this retrospective st...

BACKGROUND Fetal DNA in maternal urine, if present, would be a valuable source of fetal genetic material for noninvasive prenatal diagnosis. However, the existence of fetal DNA in maternal urine has remained controversial. The issue is due to the lack of appropriate technology to robustly detect the potentially highly degraded fetal DNA in maternal urine. METHODOLOGY We have used massively pa...

Both intact fetal cells as well as cell-free fetal DNA are present in the maternal circulation and can be recovered for non-invasive prenatal genetic diagnosis. Although methods for enrichment and isolation of rare intact fetal cells have been challenging, diagnosis of fetal chromosomal aneuploidy including trisomy 21 in first- and second-trimester pregnancies has been achieved with a 50-75% de...

OBJECTIVE To provide an introduction to new technologies involving maternal plasma cell-free fetal DNA for non-invasive prenatal diagnosis and screening in obstetrics. OPTIONS Limited to introductory discussion of maternal plasma cell-free fetal DNA. EVIDENCE MEDLINE was searched to identify publications related to the topic after 1996. This document represents an abstraction of the informa...

OBJECTIVE Cell-free fetal DNA is a source of fetal genetic material that can be used for non-invasive prenatal diagnosis. Usually constituting less than 10% of the total cell free DNA in maternal plasma, the majority is maternal in origin. Optimizing conditions for maximizing yield of cell-free fetal DNA will be crucial for effective implementation of testing. We explore factors influencing yie...

OBJECTIVE In contrast to the traditional teaching that the placenta forms an impermeable barrier, multiple studies show that both intact fetal cells and cell-free nucleic acids circulate freely in maternal blood. Complications of pregnancy, such as pre-eclampsia, or fetal cytogenetic abnormalities, such as trisomy 21, increase transfusion of both intact fetal cells and cell-free fetal nucleic a...