Cryopreservation of semen from the Caspian brown trout (Salmo trutta caspius) and effects of extender type, freezing and thawing rates on fertilization ability were studied. After assessment of semen quality, one part of semen was diluted with three parts of different extender and decanted into 0.5ml straws. Freezing was carried out at two freezing levels, 1.5cm and 2cm above surface of liquid ...

Background Low-density lipoproteins (LDL) is known to protect boar sperm during freezing–thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing–thawing. Ma...

We evaluated the effects of time, temperature, freezing, and thawing on the cerebrospinal fluid viral load by using the Roche AMPLICOR HIV-1 MONITOR test, version 1.5 (ultrasensitive). While a statistically significant decrease from the baseline was observed at 24 h, but not at 6 or 12 h, and with one freeze-thaw cycle, all changes were within the range of intra-assay variability.

Colon bacilli, Virus III, vaccine virus, herpes virus, bacteriophage, complement, and trypsin are either killed or inactivated by repeated freezing (-185 degrees C.) and thawing. As might be expected, some of the agents are more resistant than others. Hence it may be concluded that destruction or inactivation of an active agent by repeated freezing (-185 degrees C.) and thawing is not proof tha...

The motility of sperm after freezing and thawing is critical for effective cryopreservation. It is known that supplementation with cholesterol-loaded cyclodextrin (CLC) improves cryosurvival of sperm in various animals. To clarify the effects of supplementation with CLC on rabbit sperm motility after freezing and thawing, rabbit sperm motility was analyzed using a computer-assisted sperm analys...

Hybrid fiber reinforced concrete (HFRC) consisting of two or more different types of fibers has been widely investigated because of its superior mechanical properties. In the present study, the effect of the addition of steel (0.25%, 0.5%, 0.75%, and 1% of concrete volume) and Polypropylene (0.2%, 0.4%, and 0.6% of concrete volume) fibers on the surface scaling resistance of concrete, depth of ...

Road and railway tunnels in cold regions are often affected by problems related to water leakage and freezing temperatures. Water leakage in a tunnel leads to ice growth when the temperature goes below freezing and creates favourable environment for fallouts of shotcrete and rock. This paper presents results and observations from laboratory freezing – thawing experiments on rock blocks covered ...

Freezing-thawing effects on the nuclei of porcine and human spermatozoa were studied by determining native DNA percentage from fluorescence after acridine orange (AO) staining and by analyzing chromatin structure by a quantitative microspectrophotometric study of Feulgen-DNA complexes before and after freezing. The study of boar spermatozoa revealed no alteration in native DNA percentage after ...

The ability to probe fresh tissue is a key feature to biomedical Raman spectroscopy. However, it is unclear how Raman spectra of calcified tissues are affected by freezing. In this study, six transverse sections of femoral cortical bone were subjected to multiple freeze∕thaw cycles and probed using a custom Raman microscope. Significant decreases were observed in the amide I and amide III bands...