frozen section and touch imprint cytology are important diagnostic procedures for surgeons during operation and must be accurate if the patient is to receive maximum benefit. to assess the accuracy of intraoperative pathologic consultation including frozen section and touch imprint cytology during a five year period (1995- 2000), a retrospective survey of 1000 consecutive cases of frozen sectio...

PURPOSE To examine normal human corneal epithelium in vivo and in vitro for expression and status of plasniinogcn activ:ltor inhibitor type 2 (PAI-2). METHODS Normal hiuman corneas were prepared for frozen sections and for culture of corneal keratinocytes. PAI-2 was analyzed by immunohistochemistry and western blot analysis uising antibodies that recognize all forms of PAI-2. RESULTS In viv...

PURPOSE The size and organization of stromal collagen fibrils influence the biomechanical and optical properties of the cornea and hence its function. How fibrillar structure varies with position across the cornea has not been fully characterized. The present study was designed to quantify the collagen fibril spacing and diameter across the normal human cornea and to relate this to the properti...

The decellularized cornea has received considerable attention for use as an artificial cornea. The decellularized cornea is free from cellular components and other immunogens, but maintains the integrity of the extracellular matrix. However, the ultrastructure of the decellularized cornea has yet to be demonstrated in detail. We investigated the influence of high hydrostatic pressure (HHP) on t...

PURPOSE To study the phenotypes, distribution, and morphologies of different antigen-presenting cells (APCs) in the murine cornea. METHODS Intravitreal injection of fluorescently tagged ovalbumin (OVA) or antibodies to MHC-II (I-A(d)), F4/80, CD11c, B7-1, and B7-2 was performed to label cells in the murine cornea. Light and transmission electron microscopy were used to examine corneal histolo...

The alpha 6 beta 4 integrin is a receptor involved in the interaction of epithelial cells with basement membranes. This integrin is unique among the known integrins in that its beta 4 subunit has a large cytoplasmic domain. The function of this cytoplasmic domain is not known. In this paper we show that the beta 4 subunit undergoes proteolytic processing in cultured cells and provide evidence t...

The barrier characteristics of the cornea are interrogated using impact micro-particles into ex vivo porcine cornea. Using a commercial gene gun (BioRad; PDS1000), microparticles were accelerated and made to embed in target materials: either ballistic gelatin as reference or corneal tissue. Statistical analysis penetration polydisperse spherical (5–22 μm dia.) with density 2.5 g/cc, 4.2 7.8 g/c...

Background and Objective: Intraoperative consultation by frozen section is a high – risk procedure with important consequences. Therefore, it is critical to determine efficiency of frozen section performance periodically. This study was performed to determine the accuracy of frozen section in Urmia University of Medical Sciences. Materials & Methods:Inthis cross sectional study, we compared th...

PURPOSE Human corneal endothelial cells (HCECs) are considered to be nonreplicative in vivo; however, isolated HCECs can be cultured and grown successfully, indicating that they retain proliferative capacity. This capacity to replicate tends to decrease with donor age. Cyclin-dependent kinase inhibitors (CKIs) are important negative regulators of the cell cycle. Of those CKIs, p16INK4a, p21WAF1...

Frozen section and touch imprint cytology are important diagnostic procedures for surgeons during operation and must be accurate if the patient is to receive maximum benefit. To assess the accuracy of intraoperative pathologic consultation including frozen section and touch imprint cytology during a five year period (1995- 2000), a retrospective survey of 1000 consecutive cases of frozen s...