Gas-liquid chromatography (GLC) is an effective method for the measurement of total serum cholesterol. Its use in the clinical laboratory, however, requires careful attention to many variables (flow rate, sample size, sensitivity of solvent and choice of the quantitation method, i.e. peak-height ratio vs. peak height). This report evaluates the effect of these variables on the quantitation of s...

A quantitative gas liquid chromatographic method for the determination of serum levels of perazine (10-[3'-(1''-methyl-4''-piperazinyl)-propyl]-phenothiazine) is described. Perazine is used as a neuroleptic drug. The main problem consists in optimizing the chromatographic system. A sensitivity of appr. 60--150 nmol/1 (20--50 microgram/1) serum is achieved. Examples of optimization, analyses wit...

The development of high-performance liquid chromatography (HPLC) in the years of 1965 to 1985 was undeniably a success story. However, an important limitation of the technique remained: in comparison to capillary gas chromatography the resolving power accessible in a reasonable analysis time is poor. The introduction of a range of miniaturized liquid phase techniques, exploiting either pressure...

Cuticular hydrocarbons of larvae of individual strains of the Anopheles gambiae sensu stricto were investigated using gas liquid chromatography. Biomedical discriminant analysis involving multivariate statistics suggests that there was clear hydrocarbon difference between the Gambian(G3), the Nigerian (16CSS and, its malathion resistant substrain, REFMA) and the Tanzanian (KWA) strains. The hig...

Eighteen aminoglycosidic antibiotics and their degradation products, containing 2-deoxystreptamine as a commonmoiety, were separated by gas-liquid chromatography. The largest compounds examined included the pentacyclic lividomycins A and B. These compoundswere separable from each other either as pertrimethylsilyl derivatives or N-trifluoroacetyl pertrimethylsilyl derivatives on a single column ...