Single λ-DNA molecules are detected on a nanopore-gated optofluidic chip electrically and optically. Statistical variations in the single particle trajectories are used to predict the intensity distribution of the fluorescence signals.

objective: although coronary artery disease (cad) is the leading cause of death in type 2 diabetic patients, it is frequently asymptomatic. myocardial perfusion imaging (mpi) is reported to show ischemia in a significant number of asymptomatic diabetic patients. we studied the prevalence and severity of myocardial perfusion defects in asymptomatic diabetic patients and its clinical impact. meth...

To compare gender-related normal limits for left ventricular (LV) ejection fraction (EF), end-diastolic and end-systolic volumes (EDV and ESV), obtained using two myocardial perfusion-gated single photon emission computed tomography (SPECT) quantification methods. A total of 185 patients were retrospectively selected from a consecutive series of patients examined for coronary artery disease (CA...

The behavior of ion channels and transporters is often modelled using discrete-state continuous-time Markov models. Such models are helpful for the interpretation experimental data can guide design experiments by testing specific predictions. Here, we present a computational tool that allows to create chosen complexity, calculate their predictions on macroscopic as well single-molecule scale. p...

Despite the fact that transmembrane proteins represent main therapeutic targets for decades, complete and in-depth knowledge about their biochemical pharmacological profiling is not fully available. In this regard, target-tailored small-molecule fluorescent ligands are a viable approach to fill in missing pieces of puzzle. Such tools, coupled with ability high-precision optical techniques image...

BACKGROUND Whether left ventricular function can be assessed accurately by gated single photon emission computed tomography (SPECT) in patients with myocardial infarction and severe perfusion defects is not well known. METHODS AND RESULTS Twenty-five patients with an acute myocardial infarction underwent 99mTc-labeled tetrofosmin (99mTc-tetrofosmin) gated SPECT and cine magnetic resonance ima...

Voltage-gated Na(+) channels play important functional roles in the generation of electrical excitability in most vertebrate and invertebrate species. These channels are members of a superfamily that includes voltage-gated K(+), voltage-gated Ca(2+) and cyclic-nucleotide-gated channels. There are nine genes encoding voltage-gated Na(+) channels in mammals, with a tenth homologous gene that has ...

BACKGROUND Electrocardiographic gated 13N-ammonia positron emission tomography (PET) enables simultaneous assessment of myocardial blood flow and left ventricular (LV) function. The aim of this study was to assess the accuracy of gated 13N-ammonia PET for evaluating global and regional LV function in patients with coronary artery disease (CAD) in comparison with conventional left ventriculograp...

BACKGROUND Gated Volumetric Modulated Arc Therapy (VMAT) is an emerging radiation therapy modality for treatment of tumors affected by respiratory motion. However, gating significantly prolongs the treatment time, as delivery is only activated during a single respiratory phase. To enhance the efficiency of gated VMAT delivery, a novel dual-gated VMAT (DG-VMAT) technique, in which delivery is ex...

UNLABELLED To evaluate myocardial blood flow (MBF) and cardiac function with a single dose of (13)NH(3), electrocardiographically (ECG) gated PET acquisition was performed after a dynamic PET scan was obtained. Gated blood-pool (GBP) imaging with C(15)O PET was also performed to compare the left ventricular ejection fraction (LVEF) obtained using the 2 methods. METHODS Six healthy volunteers ...