in this study escherichia coli de3 containing expression vector (pet21a) with cloned persian sturgeon growth hormone (psgh) gene was grown in 10 ml lb broth on a 150 rpm shaker, at the temperature of 37 °c. at the late log phase (determined by od standard curve) 100 μl isopropyl β-d-1-thiogalactopyranoside (iptg) was added for induction of gh synthesis. samples were taken every 2 hours and afte...

In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 &muL isopropyl &beta-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours an...

AMAcetyl-,-he,osanindases A andUwere purifled to homogeneityfromhuman placenta. 1n the imt'al step of purication, thfe enzymes were adsorbed on concanavalin ASepharose 4B and etuted from, the column with a-methyl D-mannoside. Subsequent purification steps includedc DEAE-cellulose column chromn-atography, QAE-Seph%adex Ediethyl-(2-hydroxypropyl)aminoethyl-Sephadex] column chromatography1 Sephade...

Gelonin, a ribosome-inactivating protein has been isolated from the seeds of Gelonium multifluorum of Euphorbiaceae family by two methods and the results are compared. In method-I conventional aqueous extraction, cation-exchange and gel-filtration chromatography has been used. In method-II S-Sepharose fast flow gel has been used to purify the proteins from the seed extract, followed by ammonium...

We have purified the membrane-intrinsic glycerol3-phosphate dehydrogenase from both normal and hyperthyroid rat liver mitochondria by extraction with Triton X-100, hydrophobic affinity chromatography, ion exchange chromatography, gel filtration, and FAD-linked Sepharose 4B affinity chromatography. The yields in both cases were over 20‘70, and purification ranged from 800to 650-fold in mitocho...

Two procedures are reported for the purification of lysyl hydroxylase, both procedures involving (NH4)2SO4 fractionation, affinity chromatography on concanavalin A-agarose and elution of the column with ethylene glycol. The additional steps in procedure A consist of gel filtration and chromatography on a hydroxyapatite column, and in procedure B of affinity chromatography on collagen linked to ...

Methods The three storage proteins were all purified from unpolished sesame seeds extracted at pH 7,5. The 11S globulin and the 7S vicilin were enriched by precipitation in water. Pure 11S globulin was obtained by a further precipitation with 35% ammonium sulfate followed by anion exchange chromatography. Pure 7S vicilin was obtained by adding gel filtration and anion exchange chromatography. T...

The health benefits of Dracocephalum heterophyllum are widely reported in traditional Tibetan medicines, but the chemical composition is limited, probably due to difficulties separating and purifying compounds. In this study, antioxidative phenylethanoids were isolated from an extract using medium- high-pressure liquid chromatography, coupled with on-line HPLC–1,1-diphenyl-2-picrylhydrazyl reco...