A novel procedure was developed for rapid separation of the three component enzymes of pig heart 2-oxoglutarate dehydrogenase complex by high performance liquid chromatography on a gel filtration column. The complex was dissociated and separated into two fractions of the first dihydrolipoamide succinyltransferase and a second yellow fraction within 1 h by chromatography on a preparative TSK-GEL...

Abstract A monomeric sensor, TagRFP-23-Ultramarine (TR-23-U), for effector caspase-3 was obtained. The overlap integrals of new pairs red fluorescent protein TagRFP with four chromoproteins were calculated. state the Ultramarine and TR-23-U sensor confirmed by gel filtration chromatography dynamic light scattering. Incubation showed possibility using fusion as a FRET apoptosis detection.

A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. "gold bean" with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-sepharose (Sulfopropyl-sepharose), and gel filtration by FPLC (Fast protein liquid chromatography) on Superdex 75. It dos...

Abstract The konjac polysaccharide aerogel filter rod has the functions of high filtration efficiency and cooling. biodegradation rate prepared by vacuum freeze drying machine was studied in this paper. results showed that at end degradation experiment (the 106 th day), gel reached 84.9%, which close to thin-layer chromatography grade cellulose, indicating it had good biodegradability.

Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments. Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography. Identification of the growth promoter as indole-3-ethanol was achieved by mass spectrometry, thi...

Interleukin 2 (IL-2), produced with and without co-stimulation by the Burkitt's lymphoma line Daudi, was purified 37,000-fold to apparent homogeneity from lymphocyte conditioned medium by (NH4)2SO4 precipitation, DEAE-cellulose ion-exchange chromatography, gel filtration, and chromatography on blue agarose and on Procion-red agarose. The purified IL-2 showed a 10(6) U/mg protein sp act. IL-2 pr...

The purpose of this study was to characterize the mitochondrial carnitine octanoyltransferase of beef heart. Carnitine acyltransferase activities were solubilized from isolated beef heart mitochondria using KC1 and the nonionic detergent, Triton X-100, at final concentrations of 1 M and 2%, respectively. Upon fractionation of the solubilized protein on Cibacron Blue Sepharose, two protein peaks...

Peptides having potent antioxidant activity were separated from the hydrolysate of wheat gluten by ion-exchange and gel filtration chromatography. Peptides obtained by SP Sephadex C-25 chromatography of the most active fraction (WP-3) were further separated using reversed-phase high performance liquid chromatography. The amino acid sequences of these peptides were Leu-Gln-Pro-Gly-Gln-Gly-Gln-Gl...